CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000188
UniProt Accession
Genbank Protein ID
 Y13380 
Genbank Nucleotide ID
Protein Name
 Myc box-dependent-interacting protein 1 
Protein Synonyms/Alias
 Amphiphysin II; Amphiphysin-like protein; Bridging integrator 1 
Gene Name
 Bin1 
Gene Synonyms/Alias
 Amph2; Amphl 
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
7*MAEMGSKGVTAGKIubiquitination[1]
282GSNTFTVKAQPSDSAubiquitination[1]
Reference
 [1] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113] 
Functional Description
 May be involved in regulation of synaptic vesicle endocytosis. May act as a tumor suppressor and inhibits malignant cell transformation. 
Sequence Annotation
 DOMAIN 29 276 BAR.
 DOMAIN 515 588 SH3.
 REGION 1 122 Interaction with BIN2 (By similarity).
 REGION 379 422 Clathrin-binding (By similarity).
 MOD_RES 296 296 Phosphoserine (By similarity).
 MOD_RES 298 298 Phosphoserine (By similarity).
 MOD_RES 304 304 Phosphoserine (By similarity).
 MOD_RES 308 308 Phosphothreonine (By similarity).
 MOD_RES 324 324 Phosphoserine (By similarity).
 MOD_RES 332 332 Phosphoserine (By similarity).  
Keyword
 3D-structure; Alternative splicing; Coiled coil; Complete proteome; Cytoplasm; Developmental protein; Differentiation; Direct protein sequencing; Endocytosis; Nucleus; Phosphoprotein; Reference proteome; SH3 domain; Tumor suppressor. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 588 AA 
Protein Sequence
MAEMGSKGVT AGKIASNVQK KLTRAQEKVL QKLGKADETK DEQFEQCVQN FNKQLTEGTR 60
LQKDLRTYLA SVKAMHEASK KLSECLQEVY EPEWPGRDEA NKIAENNDLL WMDYHQKLVD 120
QALLTMDTYL GQFPDIKSRI AKRGRKLVDY DSARHHYESL QTAKKKDEAK IAKPVSLLEK 180
AAPQWCQGKL QAHLVAQTNL LRNQAEEELI KAQKVFEEMN VDLQEELPSL WNSRVGFYVN 240
TFQSIAGLEE NFHKEMSKLN QNLNDVLVSL EKQHGSNTFT VKAQPSDSAP EKGNKSPSPP 300
PDGSPAATPE IRVNHEPEPA SGASPGATIP KSPSQLRKGP PVPPPPKHTP SKEMKQEQIL 360
SLFDDAFVPE ISVTTPSQFE APGPFSEQAS LLDLDFEPLP PVASPVKAPT PSGQSIPWDL 420
WEPTESQAGV LPSGEPSSAE GSFAVAWPSQ TAEPGPAQPA EASEVVGGTQ EPGETAASEA 480
TSSSLPAVVV ETFSATVNGA VEGSTTTGRL DLPPGFMFKV QAQHDYTATD TDELQLKAGD 540
VVLVIPFQNP EEQDEGWLMG VKESDWNQHK ELEKCRGVFP ENFTERVQ 588 
Gene Ontology
 GO:0043679; C:axon terminus; IDA:RGD.
 GO:0044300; C:cerebellar mossy fiber; IDA:RGD.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0008021; C:synaptic vesicle; IDA:RGD.
 GO:0043196; C:varicosity; IDA:RGD.
 GO:0006897; P:endocytosis; IEA:UniProtKB-KW.
 GO:0007275; P:multicellular organismal development; IEA:UniProtKB-KW.
 GO:0042692; P:muscle cell differentiation; IEA:Compara.
 GO:0045807; P:positive regulation of endocytosis; IMP:RGD.
 GO:0043547; P:positive regulation of GTPase activity; IDA:RGD. 
Interpro
 IPR027267; AH/BAR-dom.
 IPR003005; Amphiphysin.
 IPR003023; Amphiphysin_2.
 IPR004148; BAR_dom.
 IPR011511; SH3_2.
 IPR001452; SH3_domain. 
Pfam
 PF03114; BAR
 PF07653; SH3_2 
SMART
 SM00721; BAR
 SM00326; SH3 
PROSITE
 PS51021; BAR
 PS50002; SH3 
PRINTS
 PR01251; AMPHIPHYSIN.
 PR01253; AMPHIPHYSIN2.
 PR00452; SH3DOMAIN.