UniProt Accession

 NTF2_YEAST; P33331; D3DLQ6 

Genbank Protein ID

 U18778; AY558448; L22204; BK006939 

Genbank Nucleotide ID

 AAB64542.1; AAS56774.1; AAB49379.1; DAA07660.1 

Protein Name

 Nuclear transport factor 2 

Protein Synonyms/Alias

 NTF-2; Nuclear transport factor P10 

Gene Name

 NTF2 

Gene Synonyms/Alias

 YER009W 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
48	TSQLQGAKDIVEKLV	acetylation	[1]
48	TSQLQGAKDIVEKLV	ubiquitination	[2]
53	GAKDIVEKLVSLPFQ	acetylation	[1]
53	GAKDIVEKLVSLPFQ	ubiquitination	[2, 3]
61	LVSLPFQKVQHRITT	acetylation	[1]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [3] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047] 

Functional Description

 Facilitates protein transport into the nucleus. Interacts with various nucleoporins and with Ran-GDP. Could be part of a multicomponent system of cytosolic factors that assemble at the pore complex during nuclear import. In vitro, the NTF2-Ran- GDP association, in the presence of GTP, triggers dissociation of the karyopherin alpha-beta complex, allowing nuclear translocation of karyopherin alpha and the NLS substrate. 

Sequence Annotation

 DOMAIN 8 121 NTF2.  

Keyword

 3D-structure; Complete proteome; Cytoplasm; Protein transport; Reference proteome; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 125 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0016021; C:integral to membrane; ISM:SGD.
 GO:0005635; C:nuclear envelope; IDA:SGD.
 GO:0006606; P:protein import into nucleus; IMP:SGD. 

Interpro

 IPR002075; NTF2.
 IPR018222; Nuclear_transport_factor_2_euk. 

Pfam

 PF02136; NTF2 

SMART

  

PROSITE

 PS50177; NTF2_DOMAIN 

PRINTS