UniProt Accession

 TSA1_YEAST; P34760; D6VZE6 

Genbank Protein ID

 L14640; Z46659; BK006946 

Genbank Nucleotide ID

 AAA16374.1; CAA86627.1; DAA09870.1 

Protein Name

 Peroxiredoxin TSA1 

Protein Synonyms/Alias

 Cytoplasmic thiol peroxidase 1; cTPx 1; PRP; Thiol-specific antioxidant protein 1; Thioredoxin peroxidase 

Gene Name

 TSA1 

Gene Synonyms/Alias

 TPX1; TSA; ZRG14; YML028W 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
13	QKQAPTFKKTAVVDG	ubiquitination	[1]
14	KQAPTFKKTAVVDGV	ubiquitination	[1, 2]
29	FDEVSLDKYKGKYVV	acetylation	[3]
29	FDEVSLDKYKGKYVV	ubiquitination	[2]
89	AWTNIPRKEGGLGPI	acetylation	[4]
89	AWTNIPRKEGGLGPI	ubiquitination	[1, 2]
132	GLFIIDPKGVIRHIT	acetylation	[3]
132	GLFIIDPKGVIRHIT	ubiquitination	[1, 2]
181	TPGAATIKPTVEDSK	ubiquitination	[1]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047]
 [3] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [4] Preparative peptide isoelectric focusing as a tool for improving the identification of lysine-acetylated peptides from complex mixtures.
 Xie H, Bandhakavi S, Roe MR, Griffin TJ.
 J Proteome Res. 2007 May;6(5):2019-26. [PMID: 17397211] 

Functional Description

 Physiologically important antioxidant which constitutes an enzymatic defense against sulfur-containing radicals. Can provide protection against a thiol-containing oxidation system but not against an oxidation system without thiol. 

Sequence Annotation

 DOMAIN 3 161 Thioredoxin.
 ACT_SITE 48 48 Cysteine sulfenic acid (-SOH)
 MOD_RES 174 174 Phosphothreonine.
 DISULFID 48 48 Interchain (with C-171); in linked form
 DISULFID 48 48 Interchain (with C-84 in SRX1);
 DISULFID 171 171 Interchain (with C-48); in linked form  

Keyword

 3D-structure; Antioxidant; Complete proteome; Cytoplasm; Direct protein sequencing; Disulfide bond; Oxidoreductase; Peroxidase; Phosphoprotein; Redox-active center; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 196 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IDA:SGD.
 GO:0005844; C:polysome; IDA:SGD.
 GO:0043022; F:ribosome binding; IDA:SGD.
 GO:0008379; F:thioredoxin peroxidase activity; IDA:SGD.
 GO:0051082; F:unfolded protein binding; IMP:SGD.
 GO:0045454; P:cell redox homeostasis; IDA:SGD.
 GO:0034599; P:cellular response to oxidative stress; IDA:SGD.
 GO:0000077; P:DNA damage checkpoint; IGI:SGD.
 GO:0042262; P:DNA protection; IMP:SGD.
 GO:0006457; P:protein folding; IMP:SGD.
 GO:0001302; P:replicative cell aging; IMP:SGD.
 GO:0033194; P:response to hydroperoxide; IMP:SGD. 

Interpro

 IPR000866; AhpC/TSA.
 IPR024706; Peroxiredoxin_AhpC-typ.
 IPR019479; Peroxiredoxin_C.
 IPR012336; Thioredoxin-like_fold. 

Pfam

 PF10417; 1-cysPrx_C
 PF00578; AhpC-TSA 

SMART

  

PROSITE

 PS51352; THIOREDOXIN_2 

PRINTS