CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-021612
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Intraflagellar transport protein 122 homolog 
Protein Synonyms/Alias
 WD repeat-containing protein 10; WD repeat-containing protein 140 
Gene Name
 IFT122 
Gene Synonyms/Alias
 SPG; WDR10; WDR140 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
63VYCVAYAKDGKRFASubiquitination[1]
75FASGSADKSVIIWTSubiquitination[1]
83SVIIWTSKLEGILKYubiquitination[1]
124GLWSPEQKSVSKHKSubiquitination[1]
974YIPARFQKSIELGTLubiquitination[2]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Required for cilia formation during neuronal patterning. Acts as a negative regulator of Shh signaling. Required to recruit TULP3 to primary cilia (By similarity). 
Sequence Annotation
 REPEAT 10 50 WD 1.
 REPEAT 51 91 WD 2.
 REPEAT 93 129 WD 3.
 REPEAT 131 169 WD 4.
 REPEAT 278 317 WD 5.
 REPEAT 319 359 WD 6.
 REPEAT 512 551 WD 7.  
Keyword
 Alternative splicing; Cell projection; Ciliopathy; Cilium; Cilium biogenesis/degradation; Complete proteome; Cytoplasm; Cytoskeleton; Developmental protein; Disease mutation; Ectodermal dysplasia; Reference proteome; Repeat; WD repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1241 AA 
Protein Sequence
MRAVLTWRDK AEHCINDIAF KPDGTQLILA AGSRLLVYDT SDGTLLQPLK GHKDTVYCVA 60
YAKDGKRFAS GSADKSVIIW TSKLEGILKY THNDAIQCVS YNPITHQLAS CSSSDFGLWS 120
PEQKSVSKHK SSSKIICCSW TNDGQYLALG MFNGIISIRN KNGEEKVKIE RPGGSLSPIW 180
SICWNPSREE RNDILAVADW GQKVSFYQLS GKQIGKDRAL NFDPCCISYF TKGEYILLGG 240
SDKQVSLFTK DGVRLGTVGE QNSWVWTCQA KPDSNYVVVG CQDGTISFYQ LIFSTVHGLY 300
KDRYAYRDSM TDVIVQHLIT EQKVRIKCKE LVKKIAIYRN RLAIQLPEKI LIYELYSEDL 360
SDMHYRVKEK IIKKFECNLL VVCANHIILC QEKRLQCLSF SGVKEREWQM ESLIRYIKVI 420
GGPPGREGLL VGLKNGQILK IFVDNLFAIV LLKQATAVRC LDMSASRKKL AVVDENDTCL 480
VYDIDTKELL FQEPNANSVA WNTQCEDMLC FSGGGYLNIK ASTFPVHRQK LQGFVVGYNG 540
SKIFCLHVFS ISAVEVPQSA PMYQYLDRKL FKEAYQIACL GVTDTDWREL AMEALEGLDF 600
ETAKKAFIRV QDLRYLELIS SIEERKKRGE TNNDLFLADV FSYQGKFHEA AKLYKRSGHE 660
NLALEMYTDL CMFEYAKDFL GSGDPKETKM LITKQADWAR NIKEPKAAVE MYISAGEHVK 720
AIEICGDHGW VDMLIDIARK LDKAEREPLL LCATYLKKLD SPGYAAETYL KMGDLKSLVQ 780
LHVETQRWDE AFALGEKHPE FKDDIYMPYA QWLAENDRFE EAQKAFHKAG RQREAVQVLE 840
QLTNNAVAES RFNDAAYYYW MLSMQCLDIA QDPAQKDTML GKFYHFQRLA ELYHGYHAIH 900
RHTEDPFSVH RPETLFNISR FLLHSLPKDT PSGISKVKIL FTLAKQSKAL GAYRLARHAY 960
DKLRGLYIPA RFQKSIELGT LTIRAKPFHD SEELVPLCYR CSTNNPLLNN LGNVCINCRQ 1020
PFIFSASSYD VLHLVEFYLE EGITDEEAIS LIDLEVLRPK RDDRQLEIAN NSSQILRLVE 1080
TKDSIGDEDP FTAKLSFEQG GSEFVPVVVS RLVLRSMSRR DVLIKRWPPP LRWQYFRSLL 1140
PDASITMCPS CFQMFHSEDY ELLVLQHGCC PYCRRCKDDP GP 1182 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0030991; C:intraflagellar transport particle A; IDA:UniProtKB.
 GO:0005932; C:microtubule basal body; IEA:UniProtKB-SubCell.
 GO:0032391; C:photoreceptor connecting cilium; ISS:UniProtKB.
 GO:0048593; P:camera-type eye morphogenesis; ISS:UniProtKB.
 GO:0060830; P:ciliary receptor clustering involved in smoothened signaling pathway; IEA:Compara.
 GO:0060271; P:cilium morphogenesis; ISS:UniProtKB.
 GO:0010172; P:embryonic body morphogenesis; ISS:UniProtKB.
 GO:0042733; P:embryonic digit morphogenesis; IEA:Compara.
 GO:0035050; P:embryonic heart tube development; ISS:UniProtKB.
 GO:0035721; P:intraflagellar retrograde transport; ISS:UniProtKB.
 GO:0060173; P:limb development; ISS:UniProtKB.
 GO:0045879; P:negative regulation of smoothened signaling pathway; ISS:UniProtKB.
 GO:0001843; P:neural tube closure; ISS:UniProtKB.
 GO:0060831; P:smoothened signaling pathway involved in dorsal/ventral neural tube patterning; IEA:Compara. 
Interpro
 IPR011990; TPR-like_helical.
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR017986; WD40_repeat_dom. 
Pfam
 PF00400; WD40 
SMART
 SM00320; WD40 
PROSITE
 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 
PRINTS