UniProt Accession

 NDUB8_MOUSE; Q9D6J5; Q9D6M7 

Genbank Protein ID

 AK010177; AK013516; BC043019 

Genbank Nucleotide ID

 BAB26749.1; BAB28893.1; AAH43019.1 

Protein Name

 NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial 

Protein Synonyms/Alias

 Complex I-ASHI; CI-ASHI; NADH-ubiquinone oxidoreductase ASHI subunit 

Gene Name

 Ndufb8 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
176	ERGGDPTKEPEPVVH	acetylation	[1, 2]
176	ERGGDPTKEPEPVVH	ubiquitination	[3]

Reference

 [1] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [2] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [3] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity). 

Sequence Annotation

  

Keyword

 Complete proteome; Direct protein sequencing; Electron transport; Membrane; Mitochondrion; Mitochondrion inner membrane; Reference proteome; Respiratory chain; Transit peptide; Transmembrane; Transmembrane helix; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 186 AA 

Protein Sequence

Gene Ontology

 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005743; C:mitochondrial inner membrane; IDA:MGI.
 GO:0005747; C:mitochondrial respiratory chain complex I; IEA:InterPro.
 GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:InterPro.
 GO:0006120; P:mitochondrial electron transport, NADH to ubiquinone; IEA:InterPro. 

Interpro

 IPR008699; NADH_UbQ_OxRdtase_ASHI_su.
 IPR016551; NADH_UbQ_OxRdtase_b-cplx_su8. 

Pfam

 PF05821; NDUF_B8 

SMART

  

PROSITE

  

PRINTS