CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-016128
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 ATP-dependent RNA helicase DDX42 
Protein Synonyms/Alias
 DEAD box protein 42 
Gene Name
 Ddx42 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
50SSSSGFGKSAPPQLPacetylation[1]
145DKERKNVKGIRDDIEacetylation[1, 2]
195NPIAPSKKIIDPLPPacetylation[3]
Reference
 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [3] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337] 
Functional Description
 ATP-dependent RNA helicase. Binds to partially double- stranded RNAs (dsRNAs) in order to unwind RNA secondary structures. Unwinding is promoted in the presence of single-strand binding proteins. Mediates also RNA duplex formation thereby displacing the single-strand RNA binding protein. ATP and ADP modulate its activity: ATP binding and hydrolysis by DDX42 triggers RNA strand separation, whereas the ADP-bound form of the protein triggers annealing of complementary RNA strands. Involved in the survival of cells by interacting with TP53BP2 and thereby counteracting the apoptosis-stimulating activity of TP53BP2. Relocalizes TP53BP2 to the cytoplasm (By similarity). 
Sequence Annotation
 DOMAIN 284 459 Helicase ATP-binding.
 DOMAIN 487 632 Helicase C-terminal.
 NP_BIND 297 304 ATP (By similarity).
 REGION 739 828 Necessary for interaction with TP53BP2
 MOTIF 253 281 Q motif.
 MOTIF 407 410 DEAD box.
 MOD_RES 1 1 N-acetylmethionine (By similarity).
 MOD_RES 5 5 N6-acetyllysine (By similarity).
 MOD_RES 104 104 Phosphoserine.
 MOD_RES 109 109 Phosphoserine.
 MOD_RES 111 111 Phosphoserine.
 MOD_RES 185 185 Phosphoserine.  
Keyword
 Acetylation; Alternative splicing; ATP-binding; Coiled coil; Complete proteome; Cytoplasm; Direct protein sequencing; Helicase; Hydrolase; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; RNA-binding. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 929 AA 
Protein Sequence
MNWNKGGPGT KRGFGFGGFA ISAGKKEEAK LPQQSHSAFG AASSSSGFGK SAPPQLPSFY 60
KIGSKRANFD EENAYFEDEE EDSSNVDLPY IPAENSPTRQ QFHSKPADSD SDDDPLEAFM 120
AEVEDQAARD MKRLEEKDKE RKNVKGIRDD IEEEDDQEAY FRYMAENPTA GVVQEEEEDN 180
LEYDSDGNPI APSKKIIDPL PPIDHSEIDY PPFEKNFYNE HEEITNLTPQ QLIDLRHKLN 240
LRVSGAAPPR PGSSFAHFGF DEQLMHQIRK SEYTQPTPIQ CQGVPVALSG RDMIGIAKTG 300
SGKTAAFIWP MLIHIMDQKE LEPGDGPIAV IVCPTRELCQ QIHAECKRFG KAYNLRSVAV 360
YGGGSMWEQA KALQEGAEIV VCTPGRLIDH VKKKATNLQR VSYLVFDEAD RMFDMGFEYQ 420
VRSIASHVRP DRQTLLFSAT FRKKIEKLAR DILIDPIRVV QGDIGEANED VTQIVEILHS 480
GPSKWNWLTR RLVEFTSSGS VLLFVTKKAN AEELASNLKQ EGHNLGLLHG DMDQSERNKV 540
ISDFKKKDIP VLVATDVAAR GLDIPSIKTV INYDVARDID THTHRIGRTG RAGEKGVAYT 600
LLTPKDSNFA GDLVRNLEGA NQHVSKELLD LAMQNAWFRK SRFKGGKGKK LNIGGGGLGY 660
RERPGLGSEN SDRGNNNNVM SNYEAYKPST GAMGDRLTAM KAAFQSQYKS HFVAASLSNQ 720
KAGTSSAGAS GWTSAGSLNS VPTNSAQQGH NSPDNPMTSS TKNIPGFNNS GNISSAPVTY 780
PSIGAQGVNN TASGNNSREG IGGGNGKRER YTENRGGSRH SHGDGGNRHG DGGRHGDGYR 840
YPESGSRHTD GHRHGETRHG GSAGRHGESR GANDGRNGES RKEGFNRENK MDPKVDSSRM 900
DKVDSKTDKT PDGFAVPEPP KRKKSRWDS 929 
Gene Ontology
 GO:0015030; C:Cajal body; IEA:UniProtKB-SubCell.
 GO:0005737; C:cytoplasm; ISS:UniProtKB.
 GO:0016607; C:nuclear speck; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; ISS:UniProtKB.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0008026; F:ATP-dependent helicase activity; IEA:InterPro.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0008104; P:protein localization; ISS:UniProtKB. 
Interpro
 IPR011545; DNA/RNA_helicase_DEAD/DEAH_N.
 IPR014001; Helicase_ATP-bd.
 IPR001650; Helicase_C.
 IPR027417; P-loop_NTPase.
 IPR000629; RNA-helicase_DEAD-box_CS.
 IPR014014; RNA_helicase_DEAD_Q_motif. 
Pfam
 PF00270; DEAD
 PF00271; Helicase_C 
SMART
 SM00487; DEXDc
 SM00490; HELICc 
PROSITE
 PS00039; DEAD_ATP_HELICASE
 PS51192; HELICASE_ATP_BIND_1
 PS51194; HELICASE_CTER
 PS51195; Q_MOTIF 
PRINTS