CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-010249
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ribosomal RNA small subunit methyltransferase F 
Protein Synonyms/Alias
 16S rRNA m5C1407 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RsmF 
Gene Name
 rsmF 
Gene Synonyms/Alias
 yebU; b1835; JW5301 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
205SGEGVVRKDPDALKNacetylation[1]
333NFPFSPVKDREAGQIacetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 
Functional Description
 Specifically methylates the cytosine at position 1407 (m5C1407) of 16S rRNA. 
Sequence Annotation
 REGION 125 131 S-adenosyl-L-methionine binding (By
 ACT_SITE 247 247 Nucleophile (Potential).
 BINDING 149 149 S-adenosyl-L-methionine (By similarity).
 BINDING 176 176 S-adenosyl-L-methionine (By similarity).
 BINDING 194 194 S-adenosyl-L-methionine (By similarity).  
Keyword
 3D-structure; Complete proteome; Cytoplasm; Methyltransferase; Reference proteome; RNA-binding; rRNA processing; S-adenosyl-L-methionine; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 479 AA 
Protein Sequence
MAQHTVYFPD AFLTQMREAM PSTLSFDDFL AACQRPLRRS IRVNTLKISV ADFLQLTAPY 60
GWTLTPIPWC EEGFWIERDN EDALPLGSTA EHLSGLFYIQ EASSMLPVAA LFADGNAPQR 120
VMDVAAAPGS KTTQISARMN NEGAILANEF SASRVKVLHA NISRCGISNV ALTHFDGRVF 180
GAAVPEMFDA ILLDAPCSGE GVVRKDPDAL KNWSPESNQE IAATQRELID SAFHALRPGG 240
TLVYSTCTLN QEENEAVCLW LKETYPDAVE FLPLGDLFPG ANKALTEEGF LHVFPQIYDC 300
EGFFVARLRK TQAIPALPAP KYKVGNFPFS PVKDREAGQI RQAATGVGLN WDENLRLWQR 360
DKELWLFPVG IEALIGKVRF SRLGIKLAET HNKGYRWQHE AVIALASPDN MNAFELTPQE 420
AEEWYRGRDV YPQAAPVADD VLVTFQHQPI GLAKRIGSRL KNSYPRELVR DGKLFTGNA 479 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0009383; F:rRNA (cytosine-C5-)-methyltransferase activity; IDA:EcoCyc.
 GO:0070475; P:rRNA base methylation; IMP:EcoCyc. 
Interpro
 IPR001678; Fmu/NOL1/Nop2p.
 IPR018314; Fmu/NOL1/Nop2p_CS.
 IPR011023; Nop2p.
 IPR023267; RCMT.
 IPR023545; rRNA_ssu_MeTfrase_F. 
Pfam
 PF01189; Nol1_Nop2_Fmu 
SMART
  
PROSITE
 PS01153; NOL1_NOP2_SUN 
PRINTS
 PR02008; RCMTFAMILY.