CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018586
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Calcium/calmodulin-dependent protein kinase type 1 
Protein Synonyms/Alias
 CaM kinase I; CaM-KI; CaM kinase I alpha; CaMKI-alpha 
Gene Name
 Camk1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
259FIRHLMEKDPEKRFTacetylation[1]
Reference
 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441] 
Functional Description
 Calcium/calmodulin-dependent protein kinase that operates in the calcium-triggered CaMKK-CaMK1 signaling cascade and, upon calcium influx, regulates transcription activators activity, cell cycle, hormone production, cell differentiation, actin filament organization and neurite outgrowth. Recognizes the substrate consensus sequence [MVLIF]-x-R-x(2)-[ST]-x(3)-[MVLIF]. Regulates axonal extension and growth cone motility in hippocampal and cerebellar nerve cells. Upon NMDA receptor-mediated Ca(2+) elevation, promotes dendritic growth in hippocampal neurons and is essential in synapses for full long-term potentiation (LTP) and ERK2-dependent translational activation. Downstream of NMDA receptors, promotes the formation of spines and synapses in hippocampal neurons by phosphorylating ARHGEF7/BETAPIX on 'Ser- 673', which results in the enhancement of ARHGEF7 activity and activation of RAC1. Promotes neuronal differentiation and neurite outgrowth by activation and phosphorylation of MARK2 on 'Ser-91', 'Ser-92', 'Ser-93' and 'Ser-294'. Promotes nuclear export of HDAC5 and binding to 14-3-3 by phosphorylation of 'Ser-259' and 'Ser- 498' in the regulation of muscle cell differentiation. Regulates NUMB-mediated endocytosis by phosphorylation of NUMB on 'Ser-276' and 'Ser-295'. Involved in the regulation of basal and estrogen- stimulated migration of medulloblastoma cells through ARHGEF7/BETAPIX phosphorylation. Is required for proper activation of cyclin-D1/CDK4 complex during G1 progression in diploid fibroblasts. Plays a role in K(+) and ANG2-mediated regulation of the aldosterone synthase (CYP11B2) to produce aldosterone in the adrenal cortex. Phosphorylates EIF4G3/eIF4GII. In vitro phosphorylates CREB1, ATF1, CFTR, MYL9 and SYN1/synapsin I (By similarity). 
Sequence Annotation
 DOMAIN 20 276 Protein kinase.
 NP_BIND 26 34 ATP (By similarity).
 REGION 276 316 Autoinhibitory domain (By similarity).
 REGION 296 317 Calmodulin-binding (By similarity).
 MOTIF 315 321 Nuclear export signal (By similarity).
 ACT_SITE 141 141 Proton acceptor (By similarity).
 BINDING 49 49 ATP (By similarity).
 MOD_RES 177 177 Phosphothreonine; by CaMKK1 and CaMKK2  
Keyword
 Allosteric enzyme; ATP-binding; Calmodulin-binding; Cell cycle; Complete proteome; Cytoplasm; Developmental protein; Differentiation; Kinase; Neurogenesis; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 374 AA 
Protein Sequence
MPGAVEGPRW KQAEDIRDIY DFRDVLGTGA FSEVILAEDK RTQKLVAIKC IAKKALEGKE 60
GSMENEIAVL HKIKHPNIVA LDDIYESGGH LYLIMQLVSG GELFDRIVEK GFYTERDASR 120
LIFQVLDAVK YLHDLGIVHR DLKPENLLYY SLDEDSKIMI SDFGLSKMED PGSVLSTACG 180
TPGYVAPEVL AQKPYSKAVD CWSIGVIAYI LLCGYPPFYD ENDAKLFEQI LKAEYEFDSP 240
YWDDISDSAK DFIRHLMEKD PEKRFTCEQA LQHPWIAGDT ALDKNIHQSV SEQIKKNFAK 300
SKWKQAFNAT AVVRHMRKLQ LGTSQEGQGQ TGSHGELLTP TAGGPAAGCC CRDCCVEPGS 360
ELPPAPPPSS RAMD 374 
Gene Ontology
 GO:0005954; C:calcium- and calmodulin-dependent protein kinase complex; TAS:UniProtKB.
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004683; F:calmodulin-dependent protein kinase activity; ISS:UniProtKB.
 GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
 GO:0006913; P:nucleocytoplasmic transport; IDA:MGI.
 GO:0060999; P:positive regulation of dendritic spine development; ISS:UniProtKB.
 GO:0051149; P:positive regulation of muscle cell differentiation; IEA:Compara.
 GO:0010976; P:positive regulation of neuron projection development; ISS:UniProtKB.
 GO:0046827; P:positive regulation of protein export from nucleus; IDA:MGI.
 GO:0071902; P:positive regulation of protein serine/threonine kinase activity; ISS:UniProtKB.
 GO:0051835; P:positive regulation of synapse structural plasticity; ISS:UniProtKB.
 GO:0051147; P:regulation of muscle cell differentiation; ISS:UniProtKB.
 GO:0043393; P:regulation of protein binding; ISS:UniProtKB.
 GO:0007165; P:signal transduction; IDA:MGI. 
Interpro
 IPR020636; Ca/CaM-dep_Ca-dep_prot_Kinase.
 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR002290; Ser/Thr_dual-sp_kinase_dom.
 IPR008271; Ser/Thr_kinase_AS. 
Pfam
 PF00069; Pkinase 
SMART
 SM00220; S_TKc 
PROSITE
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 
PRINTS