CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-019158
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 WD repeat-containing protein 67 
Protein Synonyms/Alias
  
Gene Name
 WDR67 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
9QSTDLGNKESGKIWHubiquitination[1]
41NTSDYHPKVLRFLNVubiquitination[1, 2, 3]
257RIIQMPTKVRAIRHLubiquitination[1]
392KMQTRILKQDLTGDFubiquitination[1, 4]
428GYGEYPTKYRMFIWRubiquitination[1]
464VAFLNLQKKYPIKSRubiquitination[2, 3]
682PVFNQYPKFIVDYQTubiquitination[2, 3]
715TVEDMQAKVDQQRVEubiquitination[4]
729EDEAWYQKQELLRKAubiquitination[1, 2, 3, 4]
750MLLQEEEKMIQQRQRubiquitination[1]
768VKRELKVKEMHLQDAubiquitination[1]
782AARRRFLKLQQDQQEubiquitination[1]
828QLELESQKRLYEKNLubiquitination[1]
833SQKRLYEKNLTENQEubiquitination[1, 2, 3, 4]
844ENQEALAKEMRADADubiquitination[1, 2, 3]
856DADAYRRKVDLEEHMubiquitination[1]
866LEEHMFHKLIEAGETubiquitination[1, 2, 3]
877AGETQSQKTQKVIKEubiquitination[1, 4, 5]
883QKTQKVIKENLAKAEubiquitination[1]
907IQSLHKQKCDDLQRNubiquitination[1]
915CDDLQRNKCYQEVAKubiquitination[1]
1042TGQNLIKKVRNLRQRubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 
Functional Description
  
Sequence Annotation
 REPEAT 38 79 WD 1.
 REPEAT 124 162 WD 2.
 REPEAT 166 205 WD 3.
 REPEAT 211 253 WD 4.
 REPEAT 304 343 WD 5.
 DOMAIN 424 599 Rab-GAP TBC.  
Keyword
 Alternative splicing; Coiled coil; Complete proteome; Polymorphism; Reference proteome; Repeat; WD repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1066 AA 
Protein Sequence
MQSTDLGNKE SGKIWHRKPS PATRDGIIVN IIHNTSDYHP KVLRFLNVAF DGTGDCLIAG 60
DHQGNIYVFD LHGNRFNLVQ RTAQACTALA FNLRRKSEFL VALADYSIKC FDTVTKELVS 120
WMRGHESSVF SISVHASGKY AITTSSDTAQ LWDLDTFQRK RKLNIRQSVG IQKVFFLPLS 180
NTILSCFKDN SIFAWECDTL FCKYQLPAPP ESSSILYKVF AVTRDGRILA AGGKSNHLHL 240
WCLEARQLFR IIQMPTKVRA IRHLEFLPDS FDAGSNQVLG VLSQDGIMRF INMQTCKLLF 300
EIGSLDEGIS SSAISPHGRY IASIMENGSL NIYSVQALTQ EINKPPPPLV KVIEDLPKNK 360
LSSSDLKMKV TSGRVQQPAK SRESKMQTRI LKQDLTGDFE SKKNELPDGL NKKRLQILLK 420
GYGEYPTKYR MFIWRSLLQL PENHTAFSTL IDKGTHVAFL NLQKKYPIKS RKLLRVLQRT 480
LSALAHWSVI FSDTPYLPLL AFPFVKLFQN NQLICFEVIA TLIINWCQHW FEYFPNPPIN 540
ILSMIENVLA FHDKELLQHF IDHDITSQLY AWPLLETVFS EVLTREEWLK LFDNIFSNHP 600
SFLLMTVVAY NICSRTPLLS CNLKDDFEFF FHHRNNLDIN VVIRQVYHLM ETTPTDIHPD 660
SMLNVFVALT KGQYPVFNQY PKFIVDYQTQ ERERIRNDEL DYLRERQTVE DMQAKVDQQR 720
VEDEAWYQKQ ELLRKAEETR REMLLQEEEK MIQQRQRLAA VKRELKVKEM HLQDAARRRF 780
LKLQQDQQEM ELRRLDDEIG RKVYMRDREI AATARDLEMR QLELESQKRL YEKNLTENQE 840
ALAKEMRADA DAYRRKVDLE EHMFHKLIEA GETQSQKTQK VIKENLAKAE QACLNTDWQI 900
QSLHKQKCDD LQRNKCYQEV AKLLRENRRK EIEIINAMVE EEAKKWKEAE GKEFRLRSAK 960
KASALSDASR KWFLKQEINA AVEHAENPCH KEEPRFQNEQ DSSCLPRTSQ LNDSSEMDPS 1020
TQISLNRRAV EWDTTGQNLI KKVRNLRQRL TARARHRCQT PHLLAA 1066 
Gene Ontology
 GO:0005813; C:centrosome; IDA:UniProtKB.
 GO:0005097; F:Rab GTPase activator activity; IEA:InterPro.
 GO:0032851; P:positive regulation of Rab GTPase activity; IEA:GOC. 
Interpro
 IPR000195; Rab-GTPase-TBC_dom.
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR019775; WD40_repeat_CS.
 IPR017986; WD40_repeat_dom. 
Pfam
 PF00566; RabGAP-TBC
 PF00400; WD40 
SMART
 SM00320; WD40 
PROSITE
 PS50086; TBC_RABGAP
 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 
PRINTS