CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006540
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Glutaredoxin-1 
Protein Synonyms/Alias
 Thioltransferase-1; TTase-1 
Gene Name
 GLRX 
Gene Synonyms/Alias
 GRX 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
9AQEFVNCKIQPGKVVacetylation[1]
9AQEFVNCKIQPGKVVubiquitination[2]
20GKVVVFIKPTCPYCRacetylation[1, 3]
20GKVVVFIKPTCPYCRubiquitination[1, 2, 4, 5, 6]
100GELLTRLKQIGALQ*ubiquitination[5, 7]
Reference
 [1] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [3] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [7] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724
Functional Description
 Has a glutathione-disulfide oxidoreductase activity in the presence of NADPH and glutathione reductase. Reduces low molecular weight disulfides and proteins. 
Sequence Annotation
 DOMAIN 3 106 Glutaredoxin.
 MOD_RES 2 2 N-acetylalanine.
 DISULFID 23 26 Redox-active.
 DISULFID 79 83  
Keyword
 3D-structure; Acetylation; Complete proteome; Cytoplasm; Direct protein sequencing; Disulfide bond; Electron transport; Polymorphism; Redox-active center; Reference proteome; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 106 AA 
Protein Sequence
MAQEFVNCKI QPGKVVVFIK PTCPYCRRAQ EILSQLPIKQ GLLEFVDITA TNHTNEIQDY 60
LQQLTGARTV PRVFIGKDCI GGCSDLVSLQ QSGELLTRLK QIGALQ 106 
Gene Ontology
 GO:0005829; C:cytosol; TAS:UniProtKB.
 GO:0005739; C:mitochondrion; IEA:Compara.
 GO:0005634; C:nucleus; IEA:Compara.
 GO:0009055; F:electron carrier activity; IEA:InterPro.
 GO:0015038; F:glutathione disulfide oxidoreductase activity; TAS:ProtInc.
 GO:0015035; F:protein disulfide oxidoreductase activity; IEA:InterPro.
 GO:0045454; P:cell redox homeostasis; IEA:InterPro.
 GO:0022900; P:electron transport chain; IEA:UniProtKB-KW.
 GO:0015949; P:nucleobase-containing small molecule interconversion; TAS:Reactome. 
Interpro
 IPR011767; GLR_AS.
 IPR002109; Glutaredoxin.
 IPR011899; Glutaredoxin_euk/vir.
 IPR014025; Glutaredoxin_subgr.
 IPR012336; Thioredoxin-like_fold. 
Pfam
 PF00462; Glutaredoxin 
SMART
  
PROSITE
 PS00195; GLUTAREDOXIN_1
 PS51354; GLUTAREDOXIN_2 
PRINTS
 PR00160; GLUTAREDOXIN.