UniProt Accession

 DHB4_RAT; P97852; P70523; P70540 

Genbank Protein ID

 S83279; U37486; X94978 

Genbank Nucleotide ID

 AAB49519.1; AAB09724.1; CAA64427.1 

Protein Name

 Peroxisomal multifunctional enzyme type 2 

Protein Synonyms/Alias

 MFE-2; 17-beta-hydroxysteroid dehydrogenase 4; 17-beta-HSD 4; D-bifunctional protein; DBP; Multifunctional protein 2; MPF-2; (3R)-hydroxyacyl-CoA dehydrogenase; Enoyl-CoA hydratase 2; 3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholest-24-enoyl-CoA hydratase 

Gene Name

 Hsd17b4 

Gene Synonyms/Alias

 Edh17b4 

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
46	NDLGGDFKGVGKGSS	acetylation	[1]
57	KGSSAADKVVEEIRR	acetylation	[1]
81	DSVEAGEKLVKTALD	acetylation	[1]
84	EAGEKLVKTALDTFG	acetylation	[1]
139	RAAWDHMKKQNYGRI	acetylation	[1]
564	AIKVRFAKPVYPGQT	acetylation	[1]
578	TLQTEMWKEGNRIHF	acetylation	[1]
668	AKWTIDLKNGSGEVY	acetylation	[1]
730	QKLQMILKDYAKL**	acetylation	[1]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 

Functional Description

 Bifunctional enzyme acting on the peroxisomal beta- oxidation pathway for fatty acids. Catalyzes the formation of 3- ketoacyl-CoA intermediates from both straight-chain and 2-methyl- branched-chain fatty acids. 

Sequence Annotation

 DOMAIN 483 599 MaoC-like.
 DOMAIN 623 735 SCP2.
 NP_BIND 16 40 NAD.
 NP_BIND 75 76 NAD (By similarity).
 NP_BIND 164 168 NAD (By similarity).
 NP_BIND 196 199 NAD (By similarity).
 REGION 2 305 (3R)-hydroxyacyl-CoA dehydrogenase.
 REGION 321 621 Enoyl-CoA hydratase 2.
 REGION 405 406 (3R)-3-hydroxydecanoyl-CoA binding (By
 REGION 509 514 (3R)-3-hydroxydecanoyl-CoA binding (By
 MOTIF 733 735 Microbody targeting signal (Potential).
 ACT_SITE 164 164 Proton acceptor.
 BINDING 21 21 NAD; via amide nitrogen (By similarity).
 BINDING 40 40 NAD (By similarity).
 BINDING 99 99 NAD; via carbonyl oxygen (By similarity).
 BINDING 151 151 Substrate.
 BINDING 434 434 (3R)-3-hydroxydecanoyl-CoA (By
 BINDING 532 532 (3R)-3-hydroxydecanoyl-CoA; via amide
 BINDING 562 562 (3R)-3-hydroxydecanoyl-CoA; via carbonyl
 BINDING 705 705 Substrate (By similarity).
 BINDING 723 723 Substrate (By similarity).
 MOD_RES 2 2 N-acetylalanine (By similarity).
 MOD_RES 3 3 Phosphoserine (By similarity).
 MOD_RES 304 304 Phosphoserine (By similarity).
 MOD_RES 308 308 Phosphoserine (By similarity).
 MOD_RES 564 564 N6-acetyllysine (By similarity).
 MOD_RES 662 662 N6-acetyllysine (By similarity).
 MOD_RES 668 668 N6-acetyllysine (By similarity).  

Keyword

 3D-structure; Acetylation; Complete proteome; Direct protein sequencing; Fatty acid metabolism; Isomerase; Lipid metabolism; Lyase; NAD; Oxidoreductase; Peroxisome; Phosphoprotein; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 735 AA 

Protein Sequence

Gene Ontology

 GO:0005739; C:mitochondrion; IEA:Compara.
 GO:0005777; C:peroxisome; IDA:HGNC.
 GO:0033989; F:3alpha,7alpha,12alpha-trihydroxy-5beta-cholest-24-enoyl-CoA hydratase activity; IEA:EC.
 GO:0016853; F:isomerase activity; IEA:UniProtKB-KW.
 GO:0032934; F:sterol binding; IEA:InterPro.
 GO:0030283; F:testosterone dehydrogenase [NAD(P)] activity; TAS:RGD.
 GO:0008203; P:cholesterol metabolic process; NAS:RGD.
 GO:0006635; P:fatty acid beta-oxidation; IEA:UniProtKB-UniPathway.
 GO:0060009; P:Sertoli cell development; IEA:Compara.
 GO:0000038; P:very long-chain fatty acid metabolic process; IEA:Compara. 

Interpro

 IPR002198; DH_sc/Rdtase_SDR.
 IPR002347; Glc/ribitol_DH.
 IPR002539; MaoC_dom.
 IPR016040; NAD(P)-bd_dom.
 IPR003033; SCP2_sterol-bd_dom. 

Pfam

 PF00106; adh_short
 PF01575; MaoC_dehydratas
 PF02036; SCP2 

SMART

  

PROSITE

 PS00061; ADH_SHORT 

PRINTS

 PR00081; GDHRDH.
 PR00080; SDRFAMILY.