CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-025153
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 NEDD8-activating enzyme E1 catalytic subunit 
Protein Synonyms/Alias
  
Gene Name
 Uba3 
Gene Synonyms/Alias
 Ube1c 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
133NVVPHFNKIQDFNDTubiquitination[1]
246VRMLQWPKEQPFGDGubiquitination[1]
270HIQWIFQKSIERASQubiquitination[1]
293RLTQGVVKRIIPAVAubiquitination[1]
347YTFEAERKENCPACSubiquitination[1]
384NSASLQMKSPAITATubiquitination[1]
395ITATLEGKNRTLYLQubiquitination[1]
420PNLSKTLKELGLVDGubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 448 AA 
Protein Sequence
MADGEEPMAV DGGCGDTGDW EGRWNHVKKF LERSGPFTHP DFEPSTESLQ FLLDTCKVLV 60
IGAGGLGCEL LKNLALSGFR QIHVIDMDTI DVSNLNRQFL FRPKDVGRPK AEVAAEFLND 120
RVPNCNVVPH FNKIQDFNDT FYRQFHIIVC GLDSIIARRW INGMLISLLN YEDGVLDPSS 180
IVPLIDGGTE GFKGNARVIL PGMTACIECT LELYPPQVNF PMCTIASMPR LPEHCIEYVR 240
MLQWPKEQPF GDGVPLDGDD PEHIQWIFQK SIERASQYNI RGVTYRLTQG VVKRIIPAVA 300
STNAVIAAVC ATEVFKIATS AYIPLNNYLV FNDVDGLYTY TFEAERKENC PACSQLPQNI 360
QFSPSAKLQE VLDYLTNSAS LQMKSPAITA TLEGKNRTLY LQSVTSIEER TRPNLSKTLK 420
ELGLVDGQEL AVADVTTPQT VLFKLHFT 448 
Gene Ontology
 GO:0016881; F:acid-amino acid ligase activity; IEA:InterPro.
 GO:0005524; F:ATP binding; IEA:InterPro.
 GO:0019781; F:NEDD8 activating enzyme activity; TAS:MGI.
 GO:0007113; P:endomitotic cell cycle; IMP:MGI.
 GO:0045116; P:protein neddylation; IEA:InterPro.
 GO:0051726; P:regulation of cell cycle; IMP:MGI. 
Interpro
 IPR014929; E2_binding.
 IPR009036; Molybdenum_cofac_synth_MoeB.
 IPR016040; NAD(P)-bd_dom.
 IPR000594; ThiF_NAD_FAD-bd.
 IPR023318; Ub_act_enz_dom_a.
 IPR000127; UBact_repeat.
 IPR019572; Ubiquitin-activating_enzyme.
 IPR018074; UBQ-activ_enz_E1_AS. 
Pfam
 PF08825; E2_bind
 PF00899; ThiF
 PF10585; UBA_e1_thiolCys
 PF02134; UBACT 
SMART
  
PROSITE
 PS00865; UBIQUITIN_ACTIVAT_2 
PRINTS