UniProt Accession

 MDHM_RAT; P04636; Q6GSM4 

Genbank Protein ID

 X04240; BC063165 

Genbank Nucleotide ID

 CAA27812.1; AAH63165.1 

Protein Name

 Malate dehydrogenase, mitochondrial 

Protein Synonyms/Alias

  

Gene Name

 Mdh2 

Gene Synonyms/Alias

 Mor1 

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
78	IETRANVKGYLGPEQ	acetylation	[1]
91	EQLPDCLKGCDVVVI	acetylation	[1]
105	IPAGVPRKPGMTRDD	acetylation	[1]
157	ITAEVFKKHGVYNPN	acetylation	[1]
165	HGVYNPNKIFGVTTL	acetylation	[1]
185	NTFVAELKGLDPARV	acetylation	[1]
203	VIGGHAGKTIIPLIS	acetylation	[1]
215	LISQCTPKVDFPQDQ	acetylation	[1]
239	EAGTEVVKAKAGAGS	acetylation	[1]
296	STPLLLGKKGLEKNL	acetylation	[1]
297	TPLLLGKKGLEKNLG	acetylation	[1]
301	LGKKGLEKNLGIGKI	acetylation	[1]
307	EKNLGIGKITPFEEK	acetylation	[1]
314	KITPFEEKMIAEAIP	acetylation	[1]
324	AEAIPELKASIKKGE	acetylation	[1]
328	PELKASIKKGEDFVK	acetylation	[1]
329	ELKASIKKGEDFVKN	acetylation	[1]
335	KKGEDFVKNMK****	acetylation	[1]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 

Functional Description

  

Sequence Annotation

 NP_BIND 31 37 NAD (By similarity).
 NP_BIND 140 142 NAD (By similarity).
 ACT_SITE 200 200 Proton acceptor (By similarity).
 BINDING 57 57 NAD (By similarity).
 BINDING 104 104 Substrate (By similarity).
 BINDING 110 110 Substrate (By similarity).
 BINDING 117 117 NAD (By similarity).
 BINDING 142 142 Substrate (By similarity).
 BINDING 176 176 Substrate (By similarity).
 BINDING 251 251 NAD (By similarity).
 MOD_RES 157 157 N6-acetyllysine (By similarity).
 MOD_RES 165 165 N6-acetyllysine (By similarity).
 MOD_RES 185 185 N6-acetyllysine (By similarity).
 MOD_RES 239 239 N6-malonyllysine; alternate (By
 MOD_RES 239 239 N6-succinyllysine; alternate (By
 MOD_RES 301 301 N6-acetyllysine; alternate (By
 MOD_RES 301 301 N6-succinyllysine; alternate (By
 MOD_RES 307 307 N6-acetyllysine; alternate (By
 MOD_RES 307 307 N6-malonyllysine; alternate (By
 MOD_RES 314 314 N6-acetyllysine (By similarity).
 MOD_RES 328 328 N6-acetyllysine; alternate (By
 MOD_RES 328 328 N6-succinyllysine; alternate (By
 MOD_RES 329 329 N6-acetyllysine; alternate (By
 MOD_RES 329 329 N6-malonyllysine; alternate (By
 MOD_RES 335 335 N6-acetyllysine (By similarity).  

Keyword

 Acetylation; Complete proteome; Direct protein sequencing; Mitochondrion; NAD; Oxidoreductase; Reference proteome; Transit peptide; Tricarboxylic acid cycle. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 338 AA 

Protein Sequence

Gene Ontology

 GO:0005743; C:mitochondrial inner membrane; IEA:Compara.
 GO:0005759; C:mitochondrial matrix; IDA:RGD.
 GO:0005634; C:nucleus; IEA:Compara.
 GO:0005886; C:plasma membrane; IEA:Compara.
 GO:0030060; F:L-malate dehydrogenase activity; IDA:RGD.
 GO:0046554; F:malate dehydrogenase (NADP+) activity; IDA:RGD.
 GO:0043621; F:protein self-association; IDA:RGD.
 GO:0044262; P:cellular carbohydrate metabolic process; IEA:InterPro.
 GO:0006475; P:internal protein amino acid acetylation; ISS:UniProtKB.
 GO:0006108; P:malate metabolic process; IDA:RGD.
 GO:0006734; P:NADH metabolic process; IDA:RGD.
 GO:0006107; P:oxaloacetate metabolic process; IDA:RGD.
 GO:0006099; P:tricarboxylic acid cycle; IDA:RGD. 

Interpro

 IPR001557; L-lactate/malate_DH.
 IPR022383; Lactate/malate_DH_C.
 IPR001236; Lactate/malate_DH_N.
 IPR015955; Lactate_DH/Glyco_Ohase_4_C.
 IPR001252; Malate_DH_AS.
 IPR010097; Malate_DH_type1.
 IPR016040; NAD(P)-bd_dom. 

Pfam

 PF02866; Ldh_1_C
 PF00056; Ldh_1_N 

SMART

  

PROSITE

 PS00068; MDH 

PRINTS