CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018863
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 DNA repair endonuclease XPF 
Protein Synonyms/Alias
 DNA excision repair protein ERCC-4; DNA repair protein complementing XP-F cells; Xeroderma pigmentosum group F-complementing protein 
Gene Name
 ERCC4 
Gene Synonyms/Alias
 ERCC11; XPF 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
289KSLVQDLKILRTLLQacetylation[1]
452KAEEVWMKFRKEDSSubiquitination[2, 3]
753ISMSRYYKRPVLLIEubiquitination[2, 3]
Reference
 [1] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Structure-specific DNA repair endonuclease responsible for the 5-prime incision during DNA repair. Involved in homologous recombination that assists in removing interstrand cross-link. 
Sequence Annotation
 DOMAIN 683 763 ERCC4.
 REGION 233 254 Leucine-zipper 1.
 REGION 270 298 Leucine-zipper 2.
 REGION 617 916 Interaction with EME1 and ERCC1.
 MOTIF 486 491 Nuclear localization signal (Potential).
 MOD_RES 289 289 N6-acetyllysine.  
Keyword
 3D-structure; Acetylation; Complete proteome; Disease mutation; DNA damage; DNA repair; DNA-binding; Dwarfism; Endonuclease; Hydrolase; Magnesium; Nuclease; Nucleus; Polymorphism; Reference proteome; Repeat; Xeroderma pigmentosum. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 916 AA 
Protein Sequence
MESGQPARRI AMAPLLEYER QLVLELLDTD GLVVCARGLG ADRLLYHFLQ LHCHPACLVL 60
VLNTQPAEEE YFINQLKIEG VEHLPRRVTN EITSNSRYEV YTQGGVIFAT SRILVVDFLT 120
DRIPSDLITG ILVYRAHRII ESCQEAFILR LFRQKNKRGF IKAFTDNAVA FDTGFCHVER 180
VMRNLFVRKL YLWPRFHVAV NSFLEQHKPE VVEIHVSMTP TMLAIQTAIL DILNACLKEL 240
KCHNPSLEVE DLSLENAIGK PFDKTIRHYL DPLWHQLGAK TKSLVQDLKI LRTLLQYLSQ 300
YDCVTFLNLL ESLRATEKAF GQNSGWLFLD SSTSMFINAR ARVYHLPDAK MSKKEKISEK 360
MEIKEGEETK KELVLESNPK WEALTEVLKE IEAENKESEA LGGPGQVLIC ASDDRTCSQL 420
RDYITLGAEA FLLRLYRKTF EKDSKAEEVW MKFRKEDSSK RIRKSHKRPK DPQNKERAST 480
KERTLKKKKR KLTLTQMVGK PEELEEEGDV EEGYRREISS SPESCPEEIK HEEFDVNLSS 540
DAAFGILKEP LTIIHPLLGC SDPYALTRVL HEVEPRYVVL YDAELTFVRQ LEIYRASRPG 600
KPLRVYFLIY GGSTEEQRYL TALRKEKEAF EKLIREKASM VVPEEREGRD ETNLDLVRGT 660
ASADVSTDTR KAGGQEQNGT QQSIVVDMRE FRSELPSLIH RRGIDIEPVT LEVGDYILTP 720
EMCVERKSIS DLIGSLNNGR LYSQCISMSR YYKRPVLLIE FDPSKPFSLT SRGALFQEIS 780
SNDISSKLTL LTLHFPRLRI LWCPSPHATA ELFEELKQSK PQPDAATALA ITADSETLPE 840
SEKYNPGPQD FLLKMPGVNA KNCRSLMHHV KNIAELAALS QDELTSILGN AANAKQLYDF 900
IHTSFAEVVS KGKGKK 916 
Gene Ontology
 GO:0000784; C:nuclear chromosome, telomeric region; IDA:UniProtKB.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0000110; C:nucleotide-excision repair factor 1 complex; IDA:UniProtKB.
 GO:0003684; F:damaged DNA binding; IMP:UniProtKB.
 GO:0003697; F:single-stranded DNA binding; IDA:UniProtKB.
 GO:0000014; F:single-stranded DNA specific endodeoxyribonuclease activity; IBA:RefGenome.
 GO:0000724; P:double-strand break repair via homologous recombination; IMP:UniProtKB.
 GO:0000710; P:meiotic mismatch repair; IBA:RefGenome.
 GO:0032205; P:negative regulation of telomere maintenance; IMP:UniProtKB.
 GO:0000718; P:nucleotide-excision repair, DNA damage removal; TAS:Reactome.
 GO:0006295; P:nucleotide-excision repair, DNA incision, 3'-to lesion; IMP:UniProtKB.
 GO:0006296; P:nucleotide-excision repair, DNA incision, 5'-to lesion; IMP:UniProtKB.
 GO:0000712; P:resolution of meiotic recombination intermediates; IBA:RefGenome.
 GO:0009411; P:response to UV; IMP:UniProtKB.
 GO:0010834; P:telomere maintenance via telomere shortening; IMP:BHF-UCL.
 GO:0006283; P:transcription-coupled nucleotide-excision repair; TAS:Reactome.
 GO:0009650; P:UV protection; IEA:Compara. 
Interpro
 IPR020819; DNA_repair_nuc_XPF/helicase.
 IPR006166; ERCC4_domain.
 IPR006167; Rad1.
 IPR011335; Restrct_endonuc-II-like.
 IPR010994; RuvA_2-like. 
Pfam
 PF02732; ERCC4 
SMART
 SM00891; ERCC4 
PROSITE
  
PRINTS