CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002214
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Fructose-bisphosphate aldolase A 
Protein Synonyms/Alias
 Muscle-type aldolase 
Gene Name
 Aldoa 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
13PALTPEQKKELADIAacetylation[1]
14ALTPEQKKELADIAHacetylation[1]
14ALTPEQKKELADIAHubiquitination[2]
28HRIVAPGKGILAADEacetylation[1]
28HRIVAPGKGILAADEubiquitination[2]
42ESTGSIAKRLQSIGTacetylation[1]
42ESTGSIAKRLQSIGTubiquitination[2]
108KGGVVGIKVDKGVVPacetylation[1]
108KGGVVGIKVDKGVVPubiquitination[2]
111VVGIKVDKGVVPLAGacetylation[1]
111VVGIKVDKGVVPLAGubiquitination[2]
140ERCAQYKKDGADFAKubiquitination[2]
147KDGADFAKWRCVLKIacetylation[1]
147KDGADFAKWRCVLKIubiquitination[2]
153AKWRCVLKIGEHTPSacetylation[1]
200PDGDHDLKRCQYVTEacetylation[1]
230YLEGTLLKPNMVTPGacetylation[1]
294INKCPLLKPWALTFSacetylation[1]
312ALQASALKAWGGKKEacetylation[1]
312ALQASALKAWGGKKEubiquitination[2]
317ALKAWGGKKENLKAAacetylation[1]
322GGKKENLKAAQEEYIacetylation[1]
322GGKKENLKAAQEEYIubiquitination[2]
330AAQEEYIKRALANSLacetylation[1]
330AAQEEYIKRALANSLubiquitination[2]
342NSLACQGKYTPSGQSacetylation[1]
Reference
 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405]
 [2] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113
Functional Description
 Plays a key role in glycolysis and gluconeogenesis. In addition, may also function as scaffolding protein (By similarity). 
Sequence Annotation
 ACT_SITE 188 188 Proton acceptor (By similarity).
 ACT_SITE 230 230 Schiff-base intermediate with
 BINDING 56 56 Substrate.
 BINDING 147 147 Substrate.
 MOD_RES 5 5 Phosphotyrosine (By similarity).
 MOD_RES 9 9 Phosphothreonine (By similarity).
 MOD_RES 13 13 N6-acetyllysine (By similarity).
 MOD_RES 36 36 Phosphoserine (By similarity).
 MOD_RES 37 37 Phosphothreonine (By similarity).
 MOD_RES 39 39 Phosphoserine (By similarity).
 MOD_RES 42 42 N6-acetyllysine (By similarity).
 MOD_RES 46 46 Phosphoserine (By similarity).
 MOD_RES 65 65 Phosphothreonine (By similarity).
 MOD_RES 108 108 N6-acetyllysine (By similarity).
 MOD_RES 111 111 N6-malonyllysine (By similarity).
 MOD_RES 204 204 Phosphotyrosine (By similarity).
 MOD_RES 223 223 Phosphotyrosine (By similarity).
 MOD_RES 235 235 Phosphothreonine (By similarity).
 MOD_RES 241 241 Phosphothreonine (By similarity).
 MOD_RES 312 312 N6-malonyllysine (By similarity).
 MOD_RES 330 330 N6-acetyllysine (By similarity).
 MOD_RES 354 354 Phosphoserine (By similarity).
 MOD_RES 356 356 Phosphoserine (By similarity).
 MOD_RES 364 364 Phosphotyrosine (By similarity).  
Keyword
 Acetylation; Complete proteome; Direct protein sequencing; Glycolysis; Lyase; Phosphoprotein; Reference proteome; Schiff base. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 364 AA 
Protein Sequence
MPHPYPALTP EQKKELADIA HRIVAPGKGI LAADESTGSI AKRLQSIGTE NTEENRRFYR 60
QLLLTADDRV NPCIGGVILF HETLYQKADD GRPFPQVIKS KGGVVGIKVD KGVVPLAGTN 120
GETTTQGLDG LSERCAQYKK DGADFAKWRC VLKIGEHTPS SLAIMENANV LARYASICQQ 180
NGIVPIVEPE ILPDGDHDLK RCQYVTEKVL AAVYKALSDH HVYLEGTLLK PNMVTPGHAC 240
TQKFSNEEIA MATVTALRRT VPPAVPGVTF LSGGQSEEEA SINLNAINKC PLLKPWALTF 300
SYGRALQASA LKAWGGKKEN LKAAQEEYIK RALANSLACQ GKYTPSGQSG AAASESLFIS 360
NHAY 364 
Gene Ontology
 GO:0005739; C:mitochondrion; IDA:RGD.
 GO:0004332; F:fructose-bisphosphate aldolase activity; ISS:UniProtKB.
 GO:0006096; P:glycolysis; ISS:UniProtKB.
 GO:0051289; P:protein homotetramerization; ISS:UniProtKB.
 GO:0043627; P:response to estrogen stimulus; IEP:RGD.
 GO:0009408; P:response to heat; IEP:RGD.
 GO:0001666; P:response to hypoxia; IEP:RGD.
 GO:0032496; P:response to lipopolysaccharide; IEP:RGD.
 GO:0035094; P:response to nicotine; IEP:RGD. 
Interpro
 IPR000741; Aldolase_I.
 IPR013785; Aldolase_TIM. 
Pfam
 PF00274; Glycolytic 
SMART
  
PROSITE
 PS00158; ALDOLASE_CLASS_I 
PRINTS