UniProt Accession

 LEF1_HUMAN; Q9UJU2; B4DG38; B7Z8E2; E9PDK3; Q3ZCU4; Q9HAZ0 

Genbank Protein ID

 AF288571; AF198532; AF294627; AK294395; AK303272; AC092539; AC097067; AC118062; AC123576; CH471057; CH471057; BC040559; BC050632 

Genbank Nucleotide ID

 AAG01022.1; AAF13268.1; AAG26886.1; BAG57649.1; BAH13928.1; EAX06223.1; EAX06225.1; AAH40559.1; AAH50632.1 

Protein Name

 Lymphoid enhancer-binding factor 1 

Protein Synonyms/Alias

 LEF-1; T cell-specific transcription factor 1-alpha; TCF1-alpha 

Gene Name

 LEF1 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
373	SFPTCKAKAATPGPL	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling. Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1- independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells. 

Sequence Annotation

 DNA_BIND 299 367 HMG box.
 REGION 1 62 CTNNB1-binding (By similarity).
 MOD_RES 132 132 Phosphoserine.
 MOD_RES 155 155 Phosphothreonine; by NLK (Probable).
 MOD_RES 166 166 Phosphoserine; by NLK (Probable).
 CROSSLNK 27 27 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 269 269 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 Activator; Alternative promoter usage; Alternative splicing; Complete proteome; Direct protein sequencing; DNA-binding; Isopeptide bond; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Transcription; Transcription regulation; Ubl conjugation; Wnt signaling pathway. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 399 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:UniProtKB.
 GO:0032993; C:protein-DNA complex; IDA:BHF-UCL.
 GO:0005667; C:transcription factor complex; IDA:BHF-UCL.
 GO:0008013; F:beta-catenin binding; IDA:BHF-UCL.
 GO:0003682; F:chromatin binding; IBA:RefGenome.
 GO:0043027; F:cysteine-type endopeptidase inhibitor activity involved in apoptotic process; IMP:UniProtKB.
 GO:0008301; F:DNA binding, bending; ISS:UniProtKB.
 GO:0035326; F:enhancer binding; IDA:UniProtKB.
 GO:0030284; F:estrogen receptor activity; IDA:UniProtKB.
 GO:0030331; F:estrogen receptor binding; IDA:UniProtKB.
 GO:0003705; F:RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity; IBA:RefGenome.
 GO:0000977; F:RNA polymerase II regulatory region sequence-specific DNA binding; IDA:BHF-UCL.
 GO:0001228; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription; IMP:BHF-UCL.
 GO:0044212; F:transcription regulatory region DNA binding; IDA:UniProtKB.
 GO:0060033; P:anatomical structure regression; IEA:Compara.
 GO:0060561; P:apoptotic process involved in morphogenesis; IBA:RefGenome.
 GO:0042100; P:B cell proliferation; IBA:RefGenome.
 GO:0030509; P:BMP signaling pathway; IBA:RefGenome.
 GO:0060070; P:canonical Wnt receptor signaling pathway; IMP:UniProtKB.
 GO:0060326; P:cell chemotaxis; IDA:UniProtKB.
 GO:0071353; P:cellular response to interleukin-4; IDA:UniProtKB.
 GO:0060710; P:chorio-allantoic fusion; IBA:RefGenome.
 GO:0021542; P:dentate gyrus development; IBA:RefGenome.
 GO:0030326; P:embryonic limb morphogenesis; IBA:RefGenome.
 GO:0001837; P:epithelial to mesenchymal transition; ISS:BHF-UCL.
 GO:0048069; P:eye pigmentation; IBA:RefGenome.
 GO:0060325; P:face morphogenesis; IBA:RefGenome.
 GO:0021873; P:forebrain neuroblast division; IBA:RefGenome.
 GO:0021879; P:forebrain neuron differentiation; IBA:RefGenome.
 GO:0021861; P:forebrain radial glial cell differentiation; IBA:RefGenome.
 GO:0021943; P:formation of radial glial scaffolds; IBA:RefGenome.
 GO:0043966; P:histone H3 acetylation; IMP:UniProtKB.
 GO:0043967; P:histone H4 acetylation; IMP:UniProtKB.
 GO:0021854; P:hypothalamus development; IBA:RefGenome.
 GO:0006917; P:induction of apoptosis; IBA:RefGenome.
 GO:0030879; P:mammary gland development; IBA:RefGenome.
 GO:0048747; P:muscle fiber development; IBA:RefGenome.
 GO:0071866; P:negative regulation of apoptotic process in bone marrow; IMP:UniProtKB.
 GO:0090090; P:negative regulation of canonical Wnt receptor signaling pathway; IMP:UniProtKB.
 GO:0022408; P:negative regulation of cell-cell adhesion; IDA:UniProtKB.
 GO:0043392; P:negative regulation of DNA binding; IDA:UniProtKB.
 GO:0071899; P:negative regulation of estrogen receptor binding; IDA:UniProtKB.
 GO:0032696; P:negative regulation of interleukin-13 production; IDA:UniProtKB.
 GO:0032713; P:negative regulation of interleukin-4 production; IDA:UniProtKB.
 GO:0032714; P:negative regulation of interleukin-5 production; IDA:UniProtKB.
 GO:0045843; P:negative regulation of striated muscle tissue development; IEA:Compara.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IEA:Compara.
 GO:0045892; P:negative regulation of transcription, DNA-dependent; IDA:UniProtKB.
 GO:0001755; P:neural crest cell migration; IBA:RefGenome.
 GO:0030223; P:neutrophil differentiation; IMP:UniProtKB.
 GO:0071895; P:odontoblast differentiation; IBA:RefGenome.
 GO:0042475; P:odontogenesis of dentin-containing tooth; IEA:Compara.
 GO:0001649; P:osteoblast differentiation; IEP:UniProtKB.
 GO:0060021; P:palate development; ISS:BHF-UCL.
 GO:0048341; P:paraxial mesoderm formation; IBA:RefGenome.
 GO:0001569; P:patterning of blood vessels; IBA:RefGenome.
 GO:0043923; P:positive regulation by host of viral transcription; IDA:UniProtKB.
 GO:0090068; P:positive regulation of cell cycle process; IDA:UniProtKB.
 GO:0030307; P:positive regulation of cell growth; IMP:UniProtKB.
 GO:0030335; P:positive regulation of cell migration; IDA:UniProtKB.
 GO:0008284; P:positive regulation of cell proliferation; IDA:UniProtKB.
 GO:0071864; P:positive regulation of cell proliferation in bone marrow; IMP:UniProtKB.
 GO:0022409; P:positive regulation of cell-cell adhesion; IDA:UniProtKB.
 GO:0010718; P:positive regulation of epithelial to mesenchymal transition; IMP:UniProtKB.
 GO:0030854; P:positive regulation of granulocyte differentiation; IDA:UniProtKB.
 GO:0045893; P:positive regulation of transcription, DNA-dependent; IDA:UniProtKB.
 GO:0022407; P:regulation of cell-cell adhesion; IEA:Compara.
 GO:0016202; P:regulation of striated muscle tissue development; IBA:RefGenome.
 GO:0050909; P:sensory perception of taste; IEA:Compara.
 GO:0001756; P:somitogenesis; IBA:RefGenome.
 GO:0002040; P:sprouting angiogenesis; IBA:RefGenome.
 GO:0033153; P:T cell receptor V(D)J recombination; IEA:Compara.
 GO:0045063; P:T-helper 1 cell differentiation; ISS:UniProtKB.
 GO:0043586; P:tongue development; IEA:Compara.
 GO:0061153; P:trachea gland development; IBA:RefGenome. 

Interpro

 IPR027397; Catenin_binding_dom.
 IPR013558; CTNNB1-bd_N.
 IPR009071; HMG_box_dom.
 IPR024940; TCF/LEF. 

Pfam

 PF08347; CTNNB1_binding
 PF00505; HMG_box 

SMART

 SM00398; HMG 

PROSITE

 PS50118; HMG_BOX_2 

PRINTS