CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022457
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Rho guanine nucleotide exchange factor 12 
Protein Synonyms/Alias
 Leukemia-associated RhoGEF 
Gene Name
 ARHGEF12 
Gene Synonyms/Alias
 KIAA0382; LARG 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
32NRESPTDKKQKVERIacetylation[1]
Reference
 [1] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786] 
Functional Description
 May play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13). Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase and may act as GTPase-activating protein (GAP) for GNA12 and GNA13. 
Sequence Annotation
 DOMAIN 72 151 PDZ.
 DOMAIN 367 558 RGSL.
 DOMAIN 787 977 DH.
 DOMAIN 1019 1132 PH.
 MOD_RES 2 2 N-acetylserine.
 MOD_RES 22 22 Phosphoserine (By similarity).
 MOD_RES 341 341 Phosphoserine.
 MOD_RES 637 637 Phosphoserine.
 MOD_RES 736 736 Phosphothreonine.  
Keyword
 3D-structure; Acetylation; Alternative splicing; Chromosomal rearrangement; Coiled coil; Complete proteome; Cytoplasm; GTPase activation; Guanine-nucleotide releasing factor; Membrane; Phosphoprotein; Polymorphism; Proto-oncogene; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1544 AA 
Protein Sequence
MSGTQSTITD RFPLKKPIRH GSILNRESPT DKKQKVERIA SHDFDPTDSS SKKTKSSSEE 60
SRSEIYGLVQ RCVIIQKDDN GFGLTVSGDN PVFVQSVKED GAAMRAGVQT GDRIIKVNGT 120
LVTHSNHLEV VKLIKSGSYV ALTVQGRPPG SPQIPLADSE VEPSVIGHMS PIMTSPHSPG 180
ASGNMERITS PVLMGEENNV VHNQKVEILR KMLQKEQERL QLLQEDYNRT PAQRLLKEIQ 240
EAKKHIPQLQ EQLSKATGSA QDGAVVTPSR PLGDTLTVSE AETDPGDVLG RTDCSSGDAS 300
RPSSDNADSP KSGPKERIYL EENPEKSETI QDTDTQSLVG SPSTRIAPHI IGAEDDDFGT 360
EHEQINGQCS CFQSIELLKS RPAHLAVFLH HVVSQFDPAT LLCYLYSDLY KHTNSKETRR 420
IFLEFHQFFL DRSAHLKVSV PDEMSADLEK RRPELIPEDL HRHYIQTMQE RVHPEVQRHL 480
EDFRQKRSMG LTLAESELTK LDAERDKDRL TLEKERTCAE QIVAKIEEVL MTAQAVEEDK 540
SSTMQYVILM YMKHLGVKVK EPRNLEHKRG RIGFLPKIKQ SMKKDKEGEE KGKRRGFPSI 600
LGPPRRPSRH DNSAIGRAME LQKARHPKHL STPSSVSPEP QDSAKLRQSG LANEGTDAGY 660
LPANSMSSVA SGASFSQEGG KENDTGSKQV GETSAPGDTL DGTPRTLNTV FDFPPPPLDQ 720
VQEEECEVER VTEHGTPKPF RKFDSVAFGE SQSEDEQFEN DLETDPPNWQ QLVSREVLLG 780
LKPCEIKRQE VINELFYTER AHVRTLKVLD QVFYQRVSRE GILSPSELRK IFSNLEDILQ 840
LHIGLNEQMK AVRKRNETSV IDQIGEDLLT WFSGPGEEKL KHAAATFCSN QPFALEMIKS 900
RQKKDSRFQT FVQDAESNPL CRRLQLKDII PTQMQRLTKY PLLLDNIAKY TEWPTEREKV 960
KKAADHCRQI LNYVNQAVKE AENKQRLEDY QRRLDTSSLK LSEYPNVEEL RNLDLTKRKM 1020
IHEGPLVWKV NRDKTIDLYT LLLEDILVLL QKQDDRLVLR CHSKILASTA DSKHTFSPVI 1080
KLSTVLVRQV ATDNKALFVI SMSDNGAQIY ELVAQTVSEK TVWQDLICRM AASVKEQSTK 1140
PIPLPQSTPG EGDNDEEDPS KLKEEQHGIS VTGLQSPDRD LGLESTLISS KPQSHSLSTS 1200
GKSEVRDLFV AERQFAKEQH TDGTLKEVGE DYQIAIPDSH LPVSEERWAL DALRNLGLLK 1260
QLLVQQLGLT EKSVQEDWQH FPRYRTASQG PQTDSVIQNS ENIKAYHSGE GHMPFRTGTG 1320
DIATCYSPRT STESFAPRDS VGLAPQDSQA SNILVMDHMI MTPEMPTMEP EGGLDDSGEH 1380
FFDAREAHSD ENPSEGDGAV NKEEKDVNLR ISGNYLILDG YDPVQESSTD EEVASSLTLQ 1440
PMTGIPAVES THQQQHSPQN THSDGAISPF TPEFLVQQRW GAMEYSCFEI QSPSSCADSQ 1500
SQIMEYIHKI EADLEHLKKV EESYTILCQR LAGSALTDKH SDKS 1544 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
 GO:0001664; F:G-protein coupled receptor binding; IDA:MGI.
 GO:0005096; F:GTPase activator activity; IEA:UniProtKB-KW.
 GO:0005543; F:phospholipid binding; IEA:InterPro.
 GO:0005089; F:Rho guanyl-nucleotide exchange factor activity; IEA:InterPro.
 GO:0097190; P:apoptotic signaling pathway; TAS:Reactome.
 GO:0007411; P:axon guidance; TAS:Reactome.
 GO:0007186; P:G-protein coupled receptor signaling pathway; IDA:MGI.
 GO:0048011; P:neurotrophin TRK receptor signaling pathway; TAS:Reactome.
 GO:0043547; P:positive regulation of GTPase activity; IEA:GOC.
 GO:0035023; P:regulation of Rho protein signal transduction; IEA:InterPro.
 GO:0051056; P:regulation of small GTPase mediated signal transduction; TAS:Reactome.
 GO:0007264; P:small GTPase mediated signal transduction; TAS:Reactome.
 GO:0038032; P:termination of G-protein coupled receptor signaling pathway; IEA:InterPro. 
Interpro
 IPR000219; DH-domain.
 IPR001331; GDS_CDC24_CS.
 IPR001478; PDZ.
 IPR011993; PH_like_dom.
 IPR001849; Pleckstrin_homology.
 IPR015212; Regulat_G_prot_signal-like.
 IPR016137; Regulat_G_prot_signal_superfam.
 IPR015721; RhoGEF-like. 
Pfam
 PF00595; PDZ
 PF09128; RGS-like
 PF00621; RhoGEF 
SMART
 SM00228; PDZ
 SM00233; PH
 SM00325; RhoGEF 
PROSITE
 PS00741; DH_1
 PS50010; DH_2
 PS50106; PDZ
 PS50003; PH_DOMAIN 
PRINTS