CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-014300
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Cytochrome b-245 heavy chain 
Protein Synonyms/Alias
 CGD91-phox; Cytochrome b(558) subunit beta; Cytochrome b558 subunit beta; Heme-binding membrane glycoprotein gp91phox; Neutrophil cytochrome b 91 kDa polypeptide; gp91-1; gp91-phox; p22 phagocyte B-cytochrome 
Gene Name
 Cybb 
Gene Synonyms/Alias
 Cgd 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
506KDVITGLKQKTLYGRubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. Also functions as a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes. 
Sequence Annotation
 DOMAIN 54 286 Ferric oxidoreductase.
 DOMAIN 287 397 FAD-binding FR-type.
 NP_BIND 338 344 FAD (Potential).
 METAL 101 101 Iron (heme axial ligand) (Probable).
 METAL 115 115 Iron (heme axial ligand) (Probable).
 METAL 209 209 Iron (heme axial ligand) (Probable).
 METAL 222 222 Iron (heme axial ligand) (Probable).
 CARBOHYD 40 40 N-linked (GlcNAc...) (Potential).  
Keyword
 Cell membrane; Complete proteome; Electron transport; FAD; Glycoprotein; Heme; Ion channel; Ion transport; Iron; Membrane; Metal-binding; NADP; Oxidoreductase; Reference proteome; Transmembrane; Transmembrane helix; Transport; Voltage-gated channel. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 570 AA 
Protein Sequence
MGNWAVNEGL SIFVILVWLG LNVFLFINYY KVYDDGPKYN YTRKLLGSAL ALARAPAACL 60
NFNCMLILLP VCRNLLSFLR GSSACCSTRI RRQLDRNLTF HKMVAWMIAL HTAIHTIAHL 120
FNVEWCVNAR VGISDRYSIA LSDIGDNENE EYLNFAREKI KNPEGGLYVA VTRLAGITGI 180
VITLCLILII TSSTKTIRRS YFEVFWYTHH LFVIFFIGLA IHGAERIVRG QTAESLEEHN 240
LDICADKIEE WGKIKECPVP KFAGNPPMTW KWIVGPMFLY LCERLVRFWR SQQKVVITKV 300
VTHPFKTIEL QMKKKGFKME VGQYIFVKCP KVSKLEWHPF TLTSAPEEDF FSIHIRIVGD 360
WTEGLFNACG CDKQEFQDAW KLPKIAVDGP FGTASEDVFS YEVVMLVGAG IGVTPFASIL 420
KSVWYKYCDN ATSLKLKKIY FYWLCRDTHA FEWFADLLQL LETQMQERNN ANFLSYNIYL 480
TGWDESQANH FAVHHDEEKD VITGLKQKTL YGRPNWDNEF KTIASEHPNT TIGVFLCGPE 540
ALAETLSKQS ISNSESGPRG VHFIFNKENF 570 
Gene Ontology
 GO:0005887; C:integral to plasma membrane; IEA:Compara.
 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0005886; C:plasma membrane; IDA:MGI.
 GO:0009055; F:electron carrier activity; IEA:Compara.
 GO:0050660; F:flavin adenine dinucleotide binding; IEA:Compara.
 GO:0020037; F:heme binding; IEA:Compara.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0016175; F:superoxide-generating NADPH oxidase activity; IEA:Compara.
 GO:0005244; F:voltage-gated ion channel activity; IEA:UniProtKB-KW.
 GO:0022900; P:electron transport chain; IEA:UniProtKB-KW.
 GO:0050665; P:hydrogen peroxide biosynthetic process; IMP:MGI.
 GO:0042554; P:superoxide anion generation; IEA:Compara. 
Interpro
 IPR000778; Cyt_b245_heavy_chain.
 IPR013112; FAD-bd_8.
 IPR017927; Fd_Rdtase_FAD-bd.
 IPR013130; Fe3_Rdtase_TM_dom.
 IPR013121; Fe_red_NAD-bd_6.
 IPR017938; Riboflavin_synthase-like_b-brl. 
Pfam
 PF08022; FAD_binding_8
 PF01794; Ferric_reduct
 PF08030; NAD_binding_6 
SMART
  
PROSITE
 PS51384; FAD_FR 
PRINTS
 PR00466; GP91PHOX.