CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-020367
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Polycomb group RING finger protein 6 
Protein Synonyms/Alias
 Mel18 and Bmi1-like RING finger; RING finger protein 134 
Gene Name
 PCGF6 
Gene Synonyms/Alias
 MBLR; RNF134 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
239SSKGRSKKVLESVFRubiquitination[1, 2]
290ATIGHVEKFLRRKMGubiquitination[1, 2, 3, 4, 5]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 Transcriptional repressor. May modulate the levels of histone H3K4Me3 by activating KDM5D histone demethylase. Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. 
Sequence Annotation
 ZN_FING 134 173 RING-type.
 MOD_RES 30 30 Phosphoserine.
 MOD_RES 115 115 Phosphoserine.  
Keyword
 3D-structure; Alternative splicing; Coiled coil; Complete proteome; Metal-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repressor; Transcription; Transcription regulation; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 350 AA 
Protein Sequence
MEGVAVVTAG SVGAAKTEGA AALPPPPPVS PPALTPAPAA GEEGPAPLSE TGAPGCSGSR 60
PPELEPERSL GRFRGRFEDE DEELEEEEEL EEEEEEEEED MSHFSLRLEG GRQDSEDEEE 120
RLINLSELTP YILCSICKGY LIDATTITEC LHTFCKSCIV RHFYYSNRCP KCNIVVHQTQ 180
PLYNIRLDRQ LQDIVYKLVI NLEEREKKQM HDFYKERGLE VPKPAVPQPV PSSKGRSKKV 240
LESVFRIPPE LDMSLLLEFI GANEGTGHFK PLEKKFVRVS GEATIGHVEK FLRRKMGLDP 300
ACQVDIICGD HLLEQYQTLR EIRRAIGDAA MQDGLLVLHY GLVVSPLKIT 350 
Gene Ontology
 GO:0035102; C:PRC1 complex; IDA:UniProtKB.
 GO:0003677; F:DNA binding; IDA:UniProtKB.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; IDA:UniProtKB.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0045892; P:negative regulation of transcription, DNA-dependent; IDA:UniProtKB.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR018957; Znf_C3HC4_RING-type.
 IPR001841; Znf_RING.
 IPR013083; Znf_RING/FYVE/PHD.
 IPR017907; Znf_RING_CS. 
Pfam
 PF00097; zf-C3HC4 
SMART
 SM00184; RING 
PROSITE
 PS00518; ZF_RING_1
 PS50089; ZF_RING_2 
PRINTS