CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-031021
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Small G protein-signaling modulator 3 
Protein Synonyms/Alias
 cDNA FLJ53139, highly similar to Homo sapiens RUN and TBC1 domain containing 3 (RUTBC3), mRNA 
Gene Name
 SGSM3 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
338RRRTQRRKSTITALLubiquitination[1]
355EDDLEALKAKNIKQTubiquitination[1, 2, 3]
385HFQCTDPKNCSVELTubiquitination[1]
419RSHRRRAKALLDFERubiquitination[1]
465LRGWFPAKFVEVLDEubiquitination[1, 2, 3]
475EVLDERSKEYSIAGDubiquitination[1, 2, 3, 4]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome; SH3 domain. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 596 AA 
Protein Sequence
MEDAPQRLRW QAHLEFTHNH DVGDLTWDKI AVSLPRSEKL RSLVLAGIPH GMRPQLWMRL 60
SGALQKKRNS ELSYREIVKN SSNDETIAAK QIEKDLLRTM PSNACFASMG SIGVPRLRRV 120
LRALAWLYPE IGYCQGTGMV AACLLLFLEE EDAFWMMSAI IEDLLPASYF STTLLGVQTD 180
QRVLRHLIVQ YLPRLDKLLQ EHDIELSLIT LHWFLTAFAS VVDIKLLLRI WDLFFYEGSR 240
VLFQLTLGML HLKEEELIQS ENSASIFNTL SDIPSQMEDA ELLLGVAMRL AGSLTDVAVE 300
TQRRKHLAYL IADQGQLLGA GTLTNLSQVV RRRTQRRKST ITALLFGEDD LEALKAKNIK 360
QTELVADLRE AILRVARHFQ CTDPKNCSVE LTPDYSMESH QRDHENYVAC SRSHRRRAKA 420
LLDFERHDDD ELGFRKNDII TIVSQKDEHC WVGELNGLRG WFPAKFVEVL DERSKEYSIA 480
GDDSVTEGVT DLVRGTLCPA LKALFEHGLK KPSLLGGACH PWLFIEEAAG REVERDFASV 540
YSRLVLCKTF RLDEDGKVLT PEELLYRAVQ SVNVTHDAVH AQMDVKLRSL ICVGLK 596 
Gene Ontology
 GO:0005622; C:intracellular; IEA:InterPro.
 GO:0005097; F:Rab GTPase activator activity; IEA:InterPro.
 GO:0032851; P:positive regulation of Rab GTPase activity; IEA:GOC. 
Interpro
 IPR000195; Rab-GTPase-TBC_dom.
 IPR004012; Run.
 IPR001452; SH3_domain. 
Pfam
 PF00566; RabGAP-TBC
 PF02759; RUN
 PF00018; SH3_1 
SMART
 SM00326; SH3
 SM00164; TBC 
PROSITE
 PS50826; RUN
 PS50002; SH3
 PS50086; TBC_RABGAP 
PRINTS