CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000867
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Sarcoplasmic/endoplasmic reticulum calcium ATPase 2 
Protein Synonyms/Alias
 SERCA2; SR Ca(2+)-ATPase 2; Calcium pump 2; Calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletal muscle isoform; Endoplasmic reticulum class 1/2 Ca(2+) ATPase 
Gene Name
 Atp2a2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
996NYLEQPGKECVQPATubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023
Functional Description
 This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Isoform SERCA2A is involved in the regulation of the contraction/relaxation cycle (By similarity). 
Sequence Annotation
 REGION 370 400 Interacts with phospholamban 1 (By
 REGION 575 594 Interacts with HAX1 (By similarity).
 REGION 787 807 Interacts with phospholamban 2 (By
 ACT_SITE 351 351 4-aspartylphosphate intermediate (By
 METAL 304 304 Calcium 2; via carbonyl oxygen (By
 METAL 305 305 Calcium 2; via carbonyl oxygen (By
 METAL 307 307 Calcium 2; via carbonyl oxygen (By
 METAL 309 309 Calcium 2 (By similarity).
 METAL 702 702 Magnesium (By similarity).
 METAL 706 706 Magnesium (By similarity).
 METAL 767 767 Calcium 1 (By similarity).
 METAL 770 770 Calcium 1 (By similarity).
 METAL 795 795 Calcium 2 (By similarity).
 METAL 798 798 Calcium 1 (By similarity).
 METAL 799 799 Calcium 1 (By similarity).
 METAL 799 799 Calcium 2 (By similarity).
 METAL 907 907 Calcium 1 (By similarity).
 MOD_RES 294 294 Nitrated tyrosine (By similarity).
 MOD_RES 295 295 Nitrated tyrosine (By similarity).
 MOD_RES 537 537 Phosphothreonine.
 MOD_RES 663 663 Phosphoserine.
 CROSSLNK 143 143 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 Alternative splicing; ATP-binding; Calcium; Calcium transport; Complete proteome; Direct protein sequencing; Endoplasmic reticulum; Hydrolase; Ion transport; Isopeptide bond; Magnesium; Membrane; Metal-binding; Nitration; Nucleotide-binding; Phosphoprotein; Reference proteome; Sarcoplasmic reticulum; Transmembrane; Transmembrane helix; Transport; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1044 AA 
Protein Sequence
MENAHTKTVE EVLGHFGVNE STGLSLEQVK KLKERWGSNE LPAEEGKTLL ELVIEQFEDL 60
LVRILLLAAC ISFVLAWFEE GEETITAFVE PFVILLILVA NAIVGVWQER NAENAIEALK 120
EYEPEMGKVY RQDRKSVQRI KAKDIVPGDI VEIAVGDKVP ADIRLTSIKS TTLRVDQSIL 180
TGESVSVIKH TDPVPDPRAV NQDKKNMLFS GTNIAAGKAM GVVVATGVNT EIGKIRDEMV 240
ATEQERTPLQ QKLDEFGEQL SKVISLICIA VWIINIGHFN DPVHGGSWIR GAIYYFKIAV 300
ALAVAAIPEG LPAVITTCLA LGTRRMAKKN AIVRSLPSVE TLGCTSVICS DKTGTLTTNQ 360
MSVCRMFILD KVEGDTCSLN EFSITGSTYA PIGEVQKDDK PVKCHQYDGL VELATICALC 420
NDSALDYNEA KGVYEKVGEA TETALTCLVE KMNVFDTELK GLSKIERANA CNSVIKQLMK 480
KEFTLEFSRD RKSMSVYCTP NKPSRTSMSK MFVKGAPEGV IDRCTHIRVG STKVPMTPGV 540
KQKIMSVIRE WGSGSDTLRC LALATHDNPL KREEMHLEDS ANFIKYETNL TFVGCVGMLD 600
PPRIEVASSV KLCRQAGIRV IMITGDNKGT AVAICRRIGI FGQDEDVTSK AFTGREFDEL 660
SPSAQRDACL NARCFARVEP SHKSKIVEFL QSFDEITAMT GDGVNDAPAL KKSEIGIAMG 720
SGTAVAKTAS EMVLADDNFS TIVAAVEEGR AIYNNMKQFI RYLISSNVGE VVCIFLTAAL 780
GFPEALIPVQ LLWVNLVTDG LPATALGFNP PDLDIMNKPP RNPKEPLISG WLFFRYLAIG 840
CYVGAATVGA AAWWFIAADG GPRVSFYQLS HFLQCKEDNP DFDGVDCAIF ESPYPMTMAL 900
SVLVTIEMCN ALNSLSENQS LLRMPPWENI WLVGSICLSM SLHFLILYVE PLPLIFQITP 960
LNLTQWLMVL KISLPVILMD ETLKFVARNY LEQPGKECVQ PATKSSCSLS ACTDGISWPF 1020
VLLIMPLVVW VYSTDTNFSD MFWS 1044 
Gene Ontology
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0016020; C:membrane; IDA:MGI.
 GO:0016529; C:sarcoplasmic reticulum; IDA:MGI.
 GO:0033017; C:sarcoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0005388; F:calcium-transporting ATPase activity; IDA:MGI.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0006874; P:cellular calcium ion homeostasis; IDA:MGI.
 GO:0006984; P:ER-nucleus signaling pathway; IMP:MGI.
 GO:0045822; P:negative regulation of heart contraction; IGI:MGI.
 GO:0006937; P:regulation of muscle contraction; TAS:MGI.
 GO:0002026; P:regulation of the force of heart contraction; IGI:MGI. 
Interpro
 IPR005782; ATPase_P-typ_Ca-transp_IIA.
 IPR006068; ATPase_P-typ_cation-transptr_C.
 IPR004014; ATPase_P-typ_cation-transptr_N.
 IPR023299; ATPase_P-typ_cyto_domN.
 IPR018303; ATPase_P-typ_P_site.
 IPR023298; ATPase_P-typ_TM_dom.
 IPR008250; ATPase_P-typ_transduc_dom_A.
 IPR001757; Cation_transp_P_typ_ATPase.
 IPR023214; HAD-like_dom. 
Pfam
 PF00689; Cation_ATPase_C
 PF00690; Cation_ATPase_N
 PF00122; E1-E2_ATPase
 PF00702; Hydrolase 
SMART
 SM00831; Cation_ATPase_N 
PROSITE
 PS00154; ATPASE_E1_E2 
PRINTS
 PR00119; CATATPASE.
 PR00120; HATPASE.