UniProt Accession

 ASGL1_RAT; Q8VI04; Q8CG44 

Genbank Protein ID

 AF329099; AJ427914; AJ427915 

Genbank Nucleotide ID

 AAL41029.1; CAD20833.1; CAD20834.1 

Protein Name

 Isoaspartyl peptidase/L-asparaginase 

Protein Synonyms/Alias

 Asparaginase-like protein 1; Asparaginase-like sperm autoantigen; Beta-aspartyl-peptidase; Glial asparaginase; Isoaspartyl dipeptidase; L-asparagine amidohydrolase; Isoaspartyl peptidase/L-asparaginase alpha chain; Isoaspartyl peptidase/L-asparaginase beta chain 

Gene Name

 Asrgl1 

Gene Synonyms/Alias

 Alp; Gliap; Hiob 

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
52	LVSEGIAKAATEGYN	acetylation	[1]
134	LARLVMEKTPHCFLT	acetylation	[1]
147	LTGRGAEKFAADMGI	acetylation	[1]
161	IPQTPAEKLITERTK	acetylation	[1]
173	RTKKHLEKEKLEKGA	acetylation	[1]
187	AQKADCPKNSGTVGA	acetylation	[1]
279	TLALDYMKSKLKGLG	acetylation	[1]
283	DYMKSKLKGLGGLIL	acetylation	[1]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 

Functional Description

 Has both L-asparaginase and beta-aspartyl peptidase activity. Is highly active with L-Asp beta-methyl ester. Besides, has catalytic activity toward beta-aspartyl dipeptides and their methyl esters, including beta-L-Asp-L-Phe, beta-L-Asp-L-Phe methyl ester (aspartame), beta-L-Asp-L-Ala, beta-L-Asp-L-Leu and beta-L- Asp-L-Lys. Does not have aspartylglucosaminidase activity and is inactive toward GlcNAc-L-Asn. Likewise, has no activity toward glutamine (By similarity). May be involved in the production of L- aspartate, which can act as an excitatory neurotransmitter in some brain regions. 

Sequence Annotation

 REGION 219 222 Substrate binding (By similarity).
 REGION 242 245 Substrate binding (By similarity).
 ACT_SITE 191 191 Nucleophile (By similarity).  

Keyword

 Autocatalytic cleavage; Complete proteome; Cytoplasm; Hydrolase; Protease; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 333 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:MGI.
 GO:0015630; C:microtubule cytoskeleton; IEA:Compara.
 GO:0005634; C:nucleus; IEA:Compara.
 GO:0004067; F:asparaginase activity; IDA:MGI.
 GO:0008798; F:beta-aspartyl-peptidase activity; ISS:UniProtKB.
 GO:0033345; P:asparagine catabolic process via L-aspartate; IDA:UniProtKB.
 GO:0006508; P:proteolysis; IEA:UniProtKB-KW. 

Interpro

 IPR000246; Peptidase_T2. 

Pfam

 PF01112; Asparaginase_2 

SMART

  

PROSITE

  

PRINTS