CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-010374
UniProt Accession
Genbank Protein ID
 U00089 
Genbank Nucleotide ID
Protein Name
 Serine hydroxymethyltransferase 
Protein Synonyms/Alias
 SHMT; Serine methylase 
Gene Name
 glyA 
Gene Synonyms/Alias
 MPN_576; MP266 
Created Date
 July 27, 2013 
Organism
 Mycoplasma pneumoniae (strain ATCC 29342 / M129) 
NCBI Taxa ID
 272634 
Lysine Modification
Position
Peptide
Type
References
240NNEAIIRKLDSGVFPacetylation[1]
266AAKYVCFKEALQPKYacetylation[1]
272FKEALQPKYKQYIQNacetylation[1]
292ASMASWFKQQGYRVIacetylation[1]
393EVKKAVLKLLERFPLacetylation[1]
Reference
 [1] Cross-talk between phosphorylation and lysine acetylation in a genome-reduced bacterium.
 van Noort V, Seebacher J, Bader S, Mohammed S, Vonkova I, Betts MJ, Kühner S, Kumar R, Maier T, O'Flaherty M, Rybin V, Schmeisky A, Yus E, Stülke J, Serrano L, Russell RB, Heck AJ, Bork P, Gavin AC.
 Mol Syst Biol. 2012 Feb 28;8:571. [PMID: 22373819
Functional Description
 Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF- independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism (By similarity). 
Sequence Annotation
 REGION 115 117 Substrate binding (By similarity).
 BINDING 25 25 Pyridoxal phosphate (By similarity).
 BINDING 45 45 Pyridoxal phosphate (By similarity).
 BINDING 47 47 Substrate (By similarity).
 BINDING 54 54 Substrate (By similarity).
 BINDING 55 55 Pyridoxal phosphate (By similarity).
 BINDING 89 89 Pyridoxal phosphate (By similarity).
 BINDING 111 111 Substrate; via carbonyl oxygen (By
 BINDING 166 166 Pyridoxal phosphate (By similarity).
 BINDING 194 194 Pyridoxal phosphate (By similarity).
 BINDING 219 219 Pyridoxal phosphate (By similarity).
 BINDING 226 226 Pyridoxal phosphate (By similarity).
 BINDING 252 252 Pyridoxal phosphate; via amide nitrogen
 BINDING 348 348 Pyridoxal phosphate (By similarity).
 MOD_RES 220 220 N6-(pyridoxal phosphate)lysine (By  
Keyword
 Amino-acid biosynthesis; Complete proteome; Cytoplasm; One-carbon metabolism; Pyridoxal phosphate; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 406 AA 
Protein Sequence
MEPKIRRILN KELQRQRDCI CLIASENYVS RDILEVTGSI LTNKYAEGYP TRRFYEGCEV 60
VDESESLAIN TCKELFGAKW ANVQPHSGSS ANYAVYLALL KPGDAILGLD LNCGGHLTHG 120
NKFNFSGKQY QPYSYTINPE TEMLDYDEVL RVAREVKPKL IICGFSNYSR TVDFERFSAI 180
AKEVGAYLLA DIAHIAGLVA AGLHPNPLPY TDVVTSTTHK TLRGPRGGLI MSNNEAIIRK 240
LDSGVFPGCQ GGPLQHVIAA KYVCFKEALQ PKYKQYIQNV KTNAASMASW FKQQGYRVIS 300
NGTDTHLFSL DVGKGKDVSQ WLQQANIVLN MNTVPFDKNP AINPSGIRIG TPAMTTRGFK 360
EKHFLYVAAL IDKIIKSDGN KKVIKEVKKA VLKLLERFPL YKGLEY 406 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0004372; F:glycine hydroxymethyltransferase activity; IEA:HAMAP.
 GO:0030170; F:pyridoxal phosphate binding; IEA:HAMAP.
 GO:0019264; P:glycine biosynthetic process from serine; IEA:HAMAP.
 GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway. 
Interpro
 IPR015424; PyrdxlP-dep_Trfase.
 IPR015421; PyrdxlP-dep_Trfase_major_sub1.
 IPR015422; PyrdxlP-dep_Trfase_major_sub2.
 IPR001085; Ser_HO-MeTrfase.
 IPR019798; Ser_HO-MeTrfase_PLP_BS. 
Pfam
 PF00464; SHMT 
SMART
  
PROSITE
 PS00096; SHMT 
PRINTS