UniProt Accession

 SRSF1_MOUSE; Q6PDM2; B2KGJ5; Q3UCH2; Q5SXC5; Q8BJV3; Q8C1H9 

Genbank Protein ID

 AK018176; AK078715; AK150535; AL593853; CU406964; BC046773; BC058627 

Genbank Nucleotide ID

 BAC25546.1; BAC37367.1; BAE29641.1; CAI24416.1; CAQ51705.1; AAH46773.1; AAH58627.1 

Protein Name

 Serine/arginine-rich splicing factor 1 

Protein Synonyms/Alias

 ASF/SF2; Pre-mRNA-splicing factor SRp30a; Splicing factor, arginine/serine-rich 1 

Gene Name

 Srsf1 

Gene Synonyms/Alias

 Sfrs1 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
30	LPPDIRTKDIEDVFY	ubiquitination	[1]
38	DIEDVFYKYGAIRDI	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Can stimulate binding of U1 snRNP to a 5'-splice site-containing pre-mRNA. Binds to purine-rich RNA sequences, either the octamer, 5'-RGAAGAAC-3' (r=A or G) or the decamers, AGGACAGAGC/AGGACGAAGC. Binds preferentially to the 5'- CGAGGCG-3' motif in vitro. Three copies of the octamer constitute a powerful splicing enhancer in vitro, the ASF/SF2 splicing enhancer (ASE) which can specifically activate ASE-dependent splicing (By similarity). Specifically regulates alternative splicing of cardiac isoforms of CAMK2D, LDB3/CYPHER and TNNT2/CTNT during heart remodeling at the juvenile to adult transition. The inappropriate accumulation of a neonatal and neuronal isoform of CAMKD2 in the adult heart results in aberrant calcium handling and defective excitation-contraction coupling in cardiomyocytes. 

Sequence Annotation

 DOMAIN 16 91 RRM 1.
 DOMAIN 121 195 RRM 2.
 REGION 198 247 Interacts with SAFB1 (By similarity).
 MOD_RES 2 2 N-acetylserine (Potential).
 MOD_RES 2 2 Phosphoserine (By similarity).
 MOD_RES 38 38 N6-acetyllysine (By similarity).
 MOD_RES 93 93 Omega-N-methylated arginine (By
 MOD_RES 97 97 Omega-N-methylated arginine (By
 MOD_RES 109 109 Omega-N-methylated arginine (By
 MOD_RES 179 179 N6-acetyllysine (By similarity).
 MOD_RES 199 199 Phosphoserine.
 MOD_RES 201 201 Phosphoserine.
 MOD_RES 205 205 Phosphoserine (By similarity).
 MOD_RES 231 231 Phosphoserine.
 MOD_RES 234 234 Phosphoserine (By similarity).
 MOD_RES 238 238 Phosphoserine.  

Keyword

 3D-structure; Acetylation; Alternative splicing; Complete proteome; Cytoplasm; Direct protein sequencing; Methylation; mRNA processing; mRNA splicing; Nucleus; Phosphoprotein; Reference proteome; Repeat; RNA-binding; Spliceosome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 248 AA 

Protein Sequence

Gene Ontology

 GO:0071013; C:catalytic step 2 spliceosome; IEA:Compara.
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0016607; C:nuclear speck; IDA:MGI.
 GO:0000166; F:nucleotide binding; IEA:InterPro.
 GO:0003723; F:RNA binding; ISS:UniProtKB.
 GO:0060048; P:cardiac muscle contraction; IGI:MGI.
 GO:0001701; P:in utero embryonic development; IMP:MGI.
 GO:0000395; P:mRNA 5'-splice site recognition; ISS:UniProtKB. 

Interpro

 IPR012677; Nucleotide-bd_a/b_plait.
 IPR000504; RRM_dom. 

Pfam

 PF00076; RRM_1 

SMART

 SM00360; RRM 

PROSITE

 PS50102; RRM 

PRINTS