UniProt Accession

 CTR9_YEAST; P89105; D6W1S4; O14409; Q07332; Q08292 

Genbank Protein ID

 U69264; U31217; Z48239; Z74887; BK006948 

Genbank Nucleotide ID

 AAB38704.1; AAB81882.1; CAA88282.1; CAA99166.1; DAA10640.2 

Protein Name

 RNA polymerase-associated protein CTR9 

Protein Synonyms/Alias

 Centromere-binding factor 1-dependent protein 1; Cln three-requiring protein 9 

Gene Name

 CTR9 

Gene Synonyms/Alias

 CDP1; YOL145C 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
196	RAKLLYQKKNYMASL	ubiquitination	[1, 2, 3]

Reference

 [1] A proteomics approach to understanding protein ubiquitination.
 Peng J, Schwartz D, Elias JE, Thoreen CC, Cheng D, Marsischky G, Roelofs J, Finley D, Gygi SP.
 Nat Biotechnol. 2003 Aug;21(8):921-6. [PMID: 12872131]
 [2] Computational identification of ubiquitylation sites from protein sequences.
 Tung CW, Ho SY.
 BMC Bioinformatics. 2008 Jul 15;9:310. [PMID: 18625080]
 [3] Identification, analysis, and prediction of protein ubiquitination sites.
 Radivojac P, Vacic V, Haynes C, Cocklin RR, Mohan A, Heyen JW, Goebl MG, Iakoucheva LM.
 Proteins. 2010 Feb 1;78(2):365-80. [PMID: 19722269] 

Functional Description

 The PAF1 complex is a multifunctional complex. Involved in transcription initiation via genetic interactions with TATA- binding proteins. Involved in elongation. It regulates 3'-end formation of snR47 by modulating the recruitment or stable association of NRD1 and NAB3 with RNA polymerase II. Also has a role in transcription-coupled histone modification. Required for activation of RAD6 ubiquitin conjugate and the BRE1 ubiquitin ligase which ubiquitinate 'Lys-126' histone H2B. Activates the SET1 histone methyltransferase complex for methylation of 'Lys-4' of histone H3 and for methylation of 'Lys-73' of histone H3 by DOT1 and 'Lys-36' of histone H3 by SET2. In complex with PAF1, required for normal CLN1 and CLN2 G1 cyclin expression in late G1. Also has a role in chromosome segregation where it appears to be involved in microtubule placement. 

Sequence Annotation

 REPEAT 56 89 TPR 1.
 REPEAT 138 174 TPR 2.
 REPEAT 183 216 TPR 3.
 REPEAT 218 251 TPR 4.
 REPEAT 298 332 TPR 5.
 REPEAT 338 371 TPR 6.
 REPEAT 373 405 TPR 7.
 REPEAT 421 455 TPR 8.
 REPEAT 462 495 TPR 9.
 REPEAT 501 534 TPR 10.
 REPEAT 540 572 TPR 11.
 REPEAT 664 697 TPR 12.
 REPEAT 699 731 TPR 13.
 REPEAT 732 764 TPR 14.
 REPEAT 768 801 TPR 15.
 REPEAT 830 863 TPR 16.
 MOD_RES 1015 1015 Phosphoserine.
 MOD_RES 1017 1017 Phosphoserine.
 CROSSLNK 196 196 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 Activator; Complete proteome; DNA-binding; Isopeptide bond; Nucleus; Phosphoprotein; Reference proteome; Repeat; TPR repeat; Transcription; Transcription regulation; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 1077 AA 

Protein Sequence

Gene Ontology

 GO:0016593; C:Cdc73/Paf1 complex; IPI:SGD.
 GO:0035327; C:transcriptionally active chromatin; IDA:SGD.
 GO:0001076; F:RNA polymerase II transcription factor binding transcription factor activity; IPI:SGD.
 GO:0045142; F:triplex DNA binding; IDA:SGD.
 GO:0031124; P:mRNA 3'-end processing; IMP:SGD.
 GO:2001255; P:positive regulation of histone H3-K36 trimethylation; IMP:SGD.
 GO:2001165; P:positive regulation of phosphorylation of RNA polymerase II C-terminal domain serine 2 residues; IMP:SGD.
 GO:2001209; P:positive regulation of transcription elongation from RNA polymerase I promoter; IDA:SGD.
 GO:0031938; P:regulation of chromatin silencing at telomere; IMP:SGD.
 GO:2001173; P:regulation of histone H2B conserved C-terminal lysine ubiquitination; IDA:SGD.
 GO:0051569; P:regulation of histone H3-K4 methylation; IMP:SGD.
 GO:0034401; P:regulation of transcription by chromatin organization; IMP:SGD.
 GO:0060260; P:regulation of transcription initiation from RNA polymerase II promoter; IMP:SGD.
 GO:0000114; P:regulation of transcription involved in G1 phase of mitotic cell cycle; IMP:SGD.
 GO:0090262; P:regulation of transcription-coupled nucleotide-excision repair; IGI:SGD.
 GO:0031126; P:snoRNA 3'-end processing; IMP:SGD.
 GO:0001015; P:snoRNA transcription from an RNA polymerase II promoter; IMP:SGD.
 GO:0006362; P:transcription elongation from RNA polymerase I promoter; IMP:SGD.
 GO:0006368; P:transcription elongation from RNA polymerase II promoter; IGI:SGD. 

Interpro

 IPR001440; TPR-1.
 IPR013026; TPR-contain_dom.
 IPR011990; TPR-like_helical.
 IPR013105; TPR_2.
 IPR019734; TPR_repeat. 

Pfam

 PF00515; TPR_1
 PF07719; TPR_2
 PF13181; TPR_8 

SMART

 SM00028; TPR 

PROSITE

 PS50005; TPR
 PS50293; TPR_REGION 

PRINTS