UniProt Accession

 EHMT2_MOUSE; Q9Z148; A2CG75; Q6PE08; Q8K4R6; Q8K4R7; Q9Z149 

Genbank Protein ID

 AF109906; AF109906; AB077209; AB077210; CT025759; BC025539; BC058357 

Genbank Nucleotide ID

 AAC84164.1; AAC84165.1; BAC05482.1; BAC05483.1; CAM27791.1; AAH25539.1; AAH58357.1 

Protein Name

 Histone-lysine N-methyltransferase EHMT2 

Protein Synonyms/Alias

 Euchromatic histone-lysine N-methyltransferase 2; HLA-B-associated transcript 8; Histone H3-K9 methyltransferase 3; H3-K9-HMTase 3; Protein G9a 

Gene Name

 Ehmt2 

Gene Synonyms/Alias

 Bat8; G9a; Ng36 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
1100	LQLYRTAKMGWGVRA	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also mediates monomethylation of 'Lys-56' of histone H3 (H3K56me1) in G1 phase, leading to promote interaction between histone H3 and PCNA and regulating DNA replication. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. May also methylate histone H1. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys- 373' of p53/TP53. Also methylates CDYL, WIZ, ACIN1, DNMT1, HDAC1, ERCC6, KLF12 and itself. 

Sequence Annotation

 REPEAT 702 731 ANK 1.
 REPEAT 737 766 ANK 2.
 REPEAT 770 799 ANK 3.
 REPEAT 803 833 ANK 4.
 REPEAT 837 866 ANK 5.
 REPEAT 870 899 ANK 6.
 REPEAT 903 932 ANK 7.
 DOMAIN 1025 1088 Pre-SET.
 DOMAIN 1091 1208 SET.
 DOMAIN 1217 1233 Post-SET.
 REGION 870 872 Histone H3K9me binding (By similarity).
 REGION 1101 1103 S-adenosyl-L-methionine binding (By
 REGION 1127 1146 Interaction with histone H3 (By
 REGION 1165 1166 S-adenosyl-L-methionine binding (By
 REGION 1207 1210 Interaction with histone H3 (By
 METAL 1027 1027 Zinc 1 (By similarity).
 METAL 1027 1027 Zinc 2 (By similarity).
 METAL 1029 1029 Zinc 1 (By similarity).
 METAL 1033 1033 Zinc 1 (By similarity).
 METAL 1033 1033 Zinc 3 (By similarity).
 METAL 1038 1038 Zinc 1 (By similarity).
 METAL 1040 1040 Zinc 2 (By similarity).
 METAL 1070 1070 Zinc 2 (By similarity).
 METAL 1070 1070 Zinc 3 (By similarity).
 METAL 1074 1074 Zinc 2 (By similarity).
 METAL 1076 1076 Zinc 3 (By similarity).
 METAL 1080 1080 Zinc 3 (By similarity).
 METAL 1168 1168 Zinc 4 (By similarity).
 METAL 1221 1221 Zinc 4 (By similarity).
 METAL 1223 1223 Zinc 4 (By similarity).
 METAL 1228 1228 Zinc 4 (By similarity).
 BINDING 1120 1120 Histone H3K9me (By similarity).
 BINDING 1138 1138 S-adenosyl-L-methionine (By similarity).
 MOD_RES 193 193 Phosphoserine (By similarity).
 MOD_RES 239 239 N6,N6,N6-trimethyllysine; by EHMT2;
 MOD_RES 239 239 N6,N6-dimethyllysine; by EHMT2; alternate
 MOD_RES 285 285 Phosphoserine (By similarity).
 MOD_RES 298 298 Phosphoserine (By similarity).
 MOD_RES 608 608 Phosphothreonine.
 MOD_RES 1263 1263 Phosphothreonine (By similarity).  

Keyword

 Alternative splicing; ANK repeat; Chromatin regulator; Chromosome; Complete proteome; Metal-binding; Methylation; Methyltransferase; Nucleus; Phosphoprotein; Reference proteome; Repeat; S-adenosyl-L-methionine; Transferase; Zinc. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 1263 AA 

Protein Sequence

Gene Ontology

 GO:0005694; C:chromosome; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0046976; F:histone methyltransferase activity (H3-K27 specific); IDA:UniProtKB.
 GO:0046974; F:histone methyltransferase activity (H3-K9 specific); IDA:UniProtKB.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0010424; P:DNA methylation on cytosine within a CG sequence; IMP:MGI.
 GO:0009566; P:fertilization; IMP:MGI.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IMP:MGI.
 GO:0035265; P:organ growth; IMP:MGI.
 GO:0018027; P:peptidyl-lysine dimethylation; ISS:UniProtKB.
 GO:0006275; P:regulation of DNA replication; IMP:UniProtKB.
 GO:0007286; P:spermatid development; IMP:MGI.
 GO:0007130; P:synaptonemal complex assembly; IMP:MGI. 

Interpro

 IPR002110; Ankyrin_rpt.
 IPR020683; Ankyrin_rpt-contain_dom.
 IPR003616; Post-SET_dom.
 IPR007728; Pre-SET_dom.
 IPR003606; Pre-SET_Zn-bd_sub.
 IPR001214; SET_dom. 

Pfam

 PF12796; Ank_2
 PF05033; Pre-SET
 PF00856; SET 

SMART

 SM00248; ANK
 SM00508; PostSET
 SM00468; PreSET
 SM00317; SET 

PROSITE

 PS50297; ANK_REP_REGION
 PS50088; ANK_REPEAT
 PS50868; POST_SET
 PS50867; PRE_SET
 PS50280; SET 

PRINTS

 PR01415; ANKYRIN.