CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012009
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 RE1-silencing transcription factor 
Protein Synonyms/Alias
 Neural-restrictive silencer factor; X2 box repressor 
Gene Name
 REST 
Gene Synonyms/Alias
 NRSF; XBR 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
280RKVYTCGKCNYFSDRubiquitination[1]
500RKSVTEVKEMDVHTGacetylation[2]
512HTGSNSEKFSKTKKSacetylation[2]
1054AKEALAVKAAKGDFVubiquitination[3]
1057ALAVKAAKGDFVCIFubiquitination[3]
Reference
 [1] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [2] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Transcriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Restricts the expression of neuronal genes by associating with two distinct corepressors, mSin3 and CoREST, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Mediates repression by recruiting the BHC complex at RE1/NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. 
Sequence Annotation
 ZN_FING 159 181 C2H2-type 1.
 ZN_FING 216 238 C2H2-type 2.
 ZN_FING 248 270 C2H2-type 3.
 ZN_FING 276 298 C2H2-type 4.
 ZN_FING 304 326 C2H2-type 5.
 ZN_FING 332 355 C2H2-type 6.
 ZN_FING 361 383 C2H2-type 7.
 ZN_FING 389 412 C2H2-type 8.
 ZN_FING 1060 1082 C2H2-type 9.
 REGION 32 122 Interaction with SIN3A.
 REGION 43 57 Interaction with SIN3B.
 REGION 1009 1087 Interaction with RCOR1.
 MOD_RES 864 864 Phosphoserine.  
Keyword
 3D-structure; Alternative splicing; Complete proteome; Metal-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Repressor; Transcription; Transcription regulation; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1097 AA 
Protein Sequence
MATQVMGQSS GGGGLFTSSG NIGMALPNDM YDLHDLSKAE LAAPQLIMLA NVALTGEVNG 60
SCCDYLVGEE RQMAELMPVG DNNFSDSEEG EGLEESADIK GEPHGLENME LRSLELSVVE 120
PQPVFEASGA PDIYSSNKDL PPETPGAEDK GKSSKTKPFR CKPCQYEAES EEQFVHHIRV 180
HSAKKFFVEE SAEKQAKARE SGSSTAEEGD FSKGPIRCDR CGYNTNRYDH YTAHLKHHTR 240
AGDNERVYKC IICTYTTVSE YHWRKHLRNH FPRKVYTCGK CNYFSDRKNN YVQHVRTHTG 300
ERPYKCELCP YSSSQKTHLT RHMRTHSGEK PFKCDQCSYV ASNQHEVTRH ARQVHNGPKP 360
LNCPHCDYKT ADRSNFKKHV ELHVNPRQFN CPVCDYAASK KCNLQYHFKS KHPTCPNKTM 420
DVSKVKLKKT KKREADLPDN ITNEKTEIEQ TKIKGDVAGK KNEKSVKAEK RDVSKEKKPS 480
NNVSVIQVTT RTRKSVTEVK EMDVHTGSNS EKFSKTKKSK RKLEVDSHSL HGPVNDEESS 540
TKKKKKVESK SKNNSQEVPK GDSKVEENKK QNTCMKKSTK KKTLKNKSSK KSSKPPQKEP 600
VEKGSAQMDP PQMGPAPTEA VQKGPVQVEP PPPMEHAQME GAQIRPAPDE PVQMEVVQEG 660
PAQKELLPPV EPAQMVGAQI VLAHMELPPP METAQTEVAQ MGPAPMEPAQ MEVAQVESAP 720
MQVVQKEPVQ MELSPPMEVV QKEPVQIELS PPMEVVQKEP VKIELSPPIE VVQKEPVQME 780
LSPPMGVVQK EPAQREPPPP REPPLHMEPI SKKPPLRKDK KEKSNMQSER ARKEQVLIEV 840
GLVPVKDSWL LKESVSTEDL SPPSPPLPKE NLREEASGDQ KLLNTGEGNK EAPLQKVGAE 900
EADESLPGLA ANINESTHIS SSGQNLNTPE GETLNGKHQT DSIVCEMKMD TDQNTRENLT 960
GINSTVEEPV SPMLPPSAVE EREAVSKTAL ASPPATMAAN ESQEIDEDEG IHSHEGSDLS 1020
DNMSEGSDDS GLHGARPVPQ ESSRKNAKEA LAVKAAKGDF VCIFCDRSFR KGKDYSKHLN 1080
RHLVNVYYLE EAAQGQE 1097 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0005829; C:cytosol; IEA:Compara.
 GO:0017053; C:transcriptional repressor complex; IDA:UniProtKB.
 GO:0003682; F:chromatin binding; ISS:UniProtKB.
 GO:0001046; F:core promoter sequence-specific DNA binding; IDA:UniProtKB.
 GO:0015271; F:outward rectifier potassium channel activity; IMP:UniProtKB.
 GO:0001078; F:RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription; IDA:UniProtKB.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0060379; P:cardiac muscle cell myoblast differentiation; ISS:UniProtKB.
 GO:0035690; P:cellular response to drug; IMP:UniProtKB.
 GO:0071257; P:cellular response to electrical stimulus; IMP:UniProtKB.
 GO:0071385; P:cellular response to glucocorticoid stimulus; IDA:UniProtKB.
 GO:0070933; P:histone H4 deacetylation; IDA:UniProtKB.
 GO:0043922; P:negative regulation by host of viral transcription; IDA:UniProtKB.
 GO:0032348; P:negative regulation of aldosterone biosynthetic process; IMP:UniProtKB.
 GO:2000798; P:negative regulation of amniotic stem cell differentiation; IMP:UniProtKB.
 GO:0045955; P:negative regulation of calcium ion-dependent exocytosis; ISS:UniProtKB.
 GO:0008285; P:negative regulation of cell proliferation; IMP:UniProtKB.
 GO:2000065; P:negative regulation of cortisol biosynthetic process; IMP:UniProtKB.
 GO:2000706; P:negative regulation of dense core granule biogenesis; ISS:UniProtKB.
 GO:0046676; P:negative regulation of insulin secretion; IMP:UniProtKB.
 GO:0050768; P:negative regulation of neurogenesis; ISS:UniProtKB.
 GO:0045665; P:negative regulation of neuron differentiation; IMP:UniProtKB.
 GO:0043065; P:positive regulation of apoptotic process; IMP:UniProtKB.
 GO:0043280; P:positive regulation of cysteine-type endopeptidase activity involved in apoptotic process; IMP:UniProtKB.
 GO:0045893; P:positive regulation of transcription, DNA-dependent; IDA:UniProtKB. 
Interpro
 IPR007087; Znf_C2H2.
 IPR015880; Znf_C2H2-like.
 IPR013087; Znf_C2H2/integrase_DNA-bd. 
Pfam
  
SMART
 SM00355; ZnF_C2H2 
PROSITE
 PS00028; ZINC_FINGER_C2H2_1
 PS50157; ZINC_FINGER_C2H2_2 
PRINTS