UniProt Accession

 GPX3_YEAST; P40581; D6VVW8 

Genbank Protein ID

 U22446; Z38061; BK006942 

Genbank Nucleotide ID

 AAA64283.1; CAA86197.1; DAA08584.1 

Protein Name

 Peroxiredoxin HYR1 

Protein Synonyms/Alias

 Glutathione peroxidase 3; Hydrogen peroxide resistance protein 1; Oxidant receptor peroxidase 1; Phospholipid hydroperoxide glutathione peroxidase 3; PHGPx3 

Gene Name

 HYR1 

Gene Synonyms/Alias

 GPX3; ORP1; YIR037W 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
50	KELEALYKRYKDEGF	acetylation	[1]
108	GNEDPVYKFLKSQKS	acetylation	[1]
114	YKFLKSQKSGMLGLR	ubiquitination	[2]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 

Functional Description

 Involved in oxidative stress response and redox homeostasis. Functions as a sensor and transducer of hydroperoxide stress. In response to hydroperoxide stress it oxidizes (activates) the transcription activator YAP1, which is involved in transcription activation of genes of the oxidative stress response pathway. May also play a direct role in hydroperoxide scavenging, being the most active of three closely related S.cerevisiae peroxiredoxins (GPX1, GPX2, and HYP1/GPX3) with respect to peroxide and lipid hydroperoxide reduction. The three enzymes are not required for the glutaredoxin-mediated antioxidant function. In the presence of peroxides, HYP1 is directly oxidized at Cys-36 to form a cysteine sulfenic acid (-SOH). Cys-36-SOH then forms either an intramolecular disulfide bond (Cys-36 with Cys-82) or a transient, intermolecular disulfide bond with 'Cys-598' of YAP1, which is further resolved into a YAP1 intramolecular disulfide bond ('Cys-303' with 'Cys-598') and a reduced Cys-36 in HYP1/GPX3. 

Sequence Annotation

 ACT_SITE 36 36 Cysteine sulfenic acid (-SOH)
 DISULFID 36 82 Redox-active.
 DISULFID 36 36 Interchain (with C-598 in YAP1);  

Keyword

 3D-structure; Complete proteome; Cytoplasm; Disulfide bond; Oxidoreductase; Peroxidase; Redox-active center; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 163 AA 

Protein Sequence

Gene Ontology

 GO:0005758; C:mitochondrial intermembrane space; IDA:SGD.
 GO:0005782; C:peroxisomal matrix; IDA:SGD.
 GO:0004602; F:glutathione peroxidase activity; IMP:SGD.
 GO:0051920; F:peroxiredoxin activity; IEA:EC.
 GO:0047066; F:phospholipid-hydroperoxide glutathione peroxidase activity; IMP:SGD.
 GO:0034599; P:cellular response to oxidative stress; IMP:SGD. 

Interpro

 IPR000889; Glutathione_peroxidase.
 IPR012336; Thioredoxin-like_fold. 

Pfam

 PF00255; GSHPx 

SMART

  

PROSITE

 PS00460; GLUTATHIONE_PEROXID_1
 PS00763; GLUTATHIONE_PEROXID_2
 PS51355; GLUTATHIONE_PEROXID_3 

PRINTS

 PR01011; GLUTPROXDASE.