UniProt Accession

 ARFG3_HUMAN; Q9NP61; E9PB03; Q9BSC6; Q9H9J0; Q9NT10; Q9NUP5; Q9Y4V3; Q9Y4V4 

Genbank Protein ID

 AF111847; AL159143; CR456382; AL049757; AL049758; AL049758; AL049757; BC005122; AL137598; AK002083; AK022768 

Genbank Nucleotide ID

 AAF40310.1; CAB76901.1; CAG30268.1; CAI21510.1; CAI21510.1; CAI20950.1; CAI20950.1; AAH05122.1; CAB70834.1; BAA92076.1; BAB14236.1 

Protein Name

 ADP-ribosylation factor GTPase-activating protein 3 

Protein Synonyms/Alias

 ARF GAP 3 

Gene Name

 ARFGAP3 

Gene Synonyms/Alias

 ARFGAP1 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
14	QDILTIFKRLRSVPT	ubiquitination	[1, 2, 3, 4]
223	KKPNQAKKGLGAKKG	acetylation	[5]
228	AKKGLGAKKGSLGAQ	acetylation	[5]
229	KKGLGAKKGSLGAQK	acetylation	[5]
336	QESPIMAKPRKKYND	ubiquitination	[3]
381	SSDSYWKKETSKDTE	ubiquitination	[3]
392	KDTETVLKTTGYSDR	ubiquitination	[1, 4]
425	QKKFGNVKAISSDMY	ubiquitination	[3]
467	DLFEEPRKQPAGNYS	ubiquitination	[3]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861] 

Functional Description

 GTPase-activating protein (GAP) for ADP ribosylation factor 1 (ARF1). Hydrolysis of ARF1-bound GTP may lead to dissociation of coatomer from Golgi-derived membranes to allow fusion with target membranes. 

Sequence Annotation

 DOMAIN 10 126 Arf-GAP.
 ZN_FING 25 48 C4-type.
 MOD_RES 231 231 Phosphoserine.
 MOD_RES 331 331 Phosphoserine.
 MOD_RES 370 370 Phosphoserine.
 MOD_RES 453 453 Phosphoserine.  

Keyword

 3D-structure; Alternative splicing; Coiled coil; Complete proteome; Cytoplasm; ER-Golgi transport; Golgi apparatus; GTPase activation; Membrane; Metal-binding; Phosphoprotein; Polymorphism; Protein transport; Reference proteome; Transport; Zinc; Zinc-finger. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 516 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0005794; C:Golgi apparatus; IDA:HPA.
 GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
 GO:0008060; F:ARF GTPase activator activity; IDA:UniProtKB.
 GO:0008565; F:protein transporter activity; NAS:UniProtKB.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0006886; P:intracellular protein transport; NAS:UniProtKB.
 GO:0009306; P:protein secretion; IEP:UniProtKB.
 GO:0032312; P:regulation of ARF GTPase activity; IEA:InterPro.
 GO:0016192; P:vesicle-mediated transport; NAS:UniProtKB. 

Interpro

 IPR001164; ArfGAP. 

Pfam

 PF01412; ArfGap 

SMART

 SM00105; ArfGap 

PROSITE

 PS50115; ARFGAP 

PRINTS

 PR00405; REVINTRACTNG.