UniProt Accession

 KLH12_HUMAN; Q53G59; A6NEN8; B7Z7B8; Q9HBX5 

Genbank Protein ID

 AF190900; AK027656; AK301791; AK223072; AC096632; BC003183; BC004175 

Genbank Nucleotide ID

 AAG17175.1; BAB55271.1; BAH13554.1; BAD96792.1; AAH03183.1; AAH04175.1 

Protein Name

 Kelch-like protein 12 

Protein Synonyms/Alias

 CUL3-interacting protein 1; DKIR homolog; hDKIR 

Gene Name

 KLHL12 

Gene Synonyms/Alias

 C3IP1 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
256	RDLVDEAKKFHLRPE	ubiquitination	[1]
301	SPIDVVEKYDPKTQE	ubiquitination	[1]
305	VVEKYDPKTQEWSFL	ubiquitination	[1, 2, 3]
462	NVTPMATKRSGAGVA	ubiquitination	[1, 2, 3, 4]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094] 

Functional Description

 Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that acts as a negative regulator of Wnt signaling pathway and ER-Golgi transport. The BCR(KLHL12) complex is involved in ER-Golgi transport by regulating the size of COPII coats, thereby playing a key role in collagen export, which is required for embryonic stem (ES) cells division: BCR(KLHL12) acts by mediating monoubiquitination of SEC31 (SEC31A or SEC31B). As part of the BCR(KLHL12) complex, also acts as a negative regulator of the Wnt signaling pathway by mediating ubiquitination and subsequent proteolysis of DVL3. The BCR(KLHL12) complex also mediates polyubiquitination of DRD4, without leading to degradation of DRD4. 

Sequence Annotation

 DOMAIN 33 100 BTB.
 DOMAIN 135 236 BACK.
 REPEAT 282 329 Kelch 1.
 REPEAT 331 379 Kelch 2.
 REPEAT 380 426 Kelch 3.
 REPEAT 427 473 Kelch 4.
 REPEAT 475 520 Kelch 5.
 REPEAT 522 567 Kelch 6.
 REGION 405 568 Interaction with DVL3.  

Keyword

 3D-structure; Alternative splicing; Complete proteome; Cytoplasmic vesicle; ER-Golgi transport; Kelch repeat; Polymorphism; Reference proteome; Repeat; Transport; Ubl conjugation pathway; Wnt signaling pathway. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 568 AA 

Protein Sequence

Gene Ontology

 GO:0031463; C:Cul3-RING ubiquitin ligase complex; IDA:UniProtKB.
 GO:0030134; C:ER to Golgi transport vesicle; IDA:UniProtKB.
 GO:0048208; P:COPII vesicle coating; IMP:UniProtKB.
 GO:0006513; P:protein monoubiquitination; IDA:UniProtKB.
 GO:0016055; P:Wnt receptor signaling pathway; IMP:UniProtKB. 

Interpro

 IPR011705; BACK.
 IPR000210; BTB/POZ-like.
 IPR011333; BTB/POZ_fold.
 IPR013069; BTB_POZ.
 IPR015916; Gal_Oxidase_b-propeller.
 IPR017096; Kelch-like_gigaxonin.
 IPR006652; Kelch_1. 

Pfam

 PF07707; BACK
 PF00651; BTB
 PF01344; Kelch_1 

SMART

 SM00875; BACK
 SM00225; BTB
 SM00612; Kelch 

PROSITE

 PS50097; BTB 

PRINTS