UniProt Accession

 RPAB3_HUMAN; P52434; P53802; Q969R0 

Genbank Protein ID

 U37689; Z49199; AJ252079; AJ252080; BC000739 

Genbank Nucleotide ID

 AAA91458.1; CAA89060.1; CAB92189.1; CAB92189.1; AAH00739.1 

Protein Name

 DNA-directed RNA polymerases I, II, and III subunit RPABC3 

Protein Synonyms/Alias

 RNA polymerases I, II, and III subunit ABC3; DNA-directed RNA polymerase II subunit H; DNA-directed RNA polymerases I, II, and III 17.1 kDa polypeptide; RPB17; RPB8 homolog; hRPB8 

Gene Name

 POLR2H 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
20	KDIDPEGKKFDRVSR	ubiquitination	[1, 2]
21	DIDPEGKKFDRVSRL	ubiquitination	[2]
95	FEYVMYGKVYRIEGD	ubiquitination	[1, 2]
146	SRVYLLMKKLAF***	ubiquitination	[1, 2, 3, 4]
147	RVYLLMKKLAF****	ubiquitination	[2, 3, 4]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 

Functional Description

 DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. 

Sequence Annotation

 REGION 16 40 Non-specific ssDNA binding.
 MOD_RES 2 2 N-acetylalanine.  

Keyword

 3D-structure; Acetylation; Complete proteome; Direct protein sequencing; DNA-binding; DNA-directed RNA polymerase; Nucleus; Reference proteome; Transcription. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 150 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0005665; C:DNA-directed RNA polymerase II, core complex; IDA:UniProtKB.
 GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0003899; F:DNA-directed RNA polymerase activity; NAS:UniProtKB.
 GO:0008270; F:zinc ion binding; NAS:UniProtKB.
 GO:0006370; P:7-methylguanosine mRNA capping; TAS:Reactome.
 GO:0000398; P:mRNA splicing, via spliceosome; TAS:Reactome.
 GO:0050434; P:positive regulation of viral transcription; TAS:Reactome.
 GO:0006363; P:termination of RNA polymerase I transcription; TAS:Reactome.
 GO:0006386; P:termination of RNA polymerase III transcription; TAS:Reactome.
 GO:0006362; P:transcription elongation from RNA polymerase I promoter; TAS:Reactome.
 GO:0006368; P:transcription elongation from RNA polymerase II promoter; TAS:Reactome.
 GO:0006385; P:transcription elongation from RNA polymerase III promoter; TAS:Reactome.
 GO:0006361; P:transcription initiation from RNA polymerase I promoter; TAS:Reactome.
 GO:0006367; P:transcription initiation from RNA polymerase II promoter; TAS:Reactome.
 GO:0006283; P:transcription-coupled nucleotide-excision repair; TAS:Reactome.
 GO:0022415; P:viral reproductive process; TAS:Reactome. 

Interpro

 IPR012340; NA-bd_OB-fold.
 IPR005570; RNA_pol_Rpb8. 

Pfam

 PF03870; RNA_pol_Rpb8 

SMART

 SM00658; RPOL8c 

PROSITE

  

PRINTS