CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-011984
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Probable E3 ubiquitin-protein ligase makorin-3 
Protein Synonyms/Alias
 RING finger protein 63; Zinc finger protein 127 
Gene Name
 MKRN3 
Gene Synonyms/Alias
 D15S9; RNF63; ZNF127 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
233QSSETERKQMAVGSGubiquitination[1]
295ACIEAHEKDMELSFAubiquitination[1]
392QKLIQQYKEAMSNKAubiquitination[1]
476RLASLLFKRFLSLRDubiquitination[2]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965] 
Functional Description
 E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins (By similarity). 
Sequence Annotation
 ZN_FING 95 122 C3H1-type 1.
 ZN_FING 238 265 C3H1-type 2.
 ZN_FING 311 365 RING-type.
 ZN_FING 394 423 C3H1-type 3.
 REGION 266 293 Makorin-type Cys-His.  
Keyword
 Complete proteome; Ligase; Metal-binding; Polymorphism; Reference proteome; Repeat; Ubl conjugation pathway; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 507 AA 
Protein Sequence
MEEPAAPSEA HEAAGAQAGA EAAREGVSGP DLPVCEPSGE SAAPDSALPH AARGWAPFPV 60
APVPAHLRRG GLRPAPASGG GAWPSPLPSR SSGIWTKQII CRYYIHGQCK EGENCRYSHD 120
LSGRKMATEG GVSPPGASAG GGPSTAAHIE PPTQEVAEAP PAASSLSLPV IGSAAERGFF 180
EAERDNADRG AAGGAGVESW ADAIEFVPGQ PYRGRWVASA PEAPLQSSET ERKQMAVGSG 240
LRFCYYASRG VCFRGESCMY LHGDICDMCG LQTLHPMDAA QREEHMRACI EAHEKDMELS 300
FAVQRGMDKV CGICMEVVYE KANPNDRRFG ILSNCNHSFC IRCIRRWRSA RQFENRIVKS 360
CPQCRVTSEL VIPSEFWVEE EEEKQKLIQQ YKEAMSNKAC RYFAEGRGNC PFGDTCFYKH 420
EYPEGWGDEP PGPGGGSFSA YWHQLVEPVR MGEGNMLYKS IKKELVVLRL ASLLFKRFLS 480
LRDELPFSED QWDLLHYELE EYFNLIL 507 
Gene Ontology
 GO:0030529; C:ribonucleoprotein complex; TAS:ProtInc.
 GO:0016874; F:ligase activity; IEA:UniProtKB-KW.
 GO:0003676; F:nucleic acid binding; IEA:InterPro.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway. 
Interpro
 IPR026290; Makorin-related.
 IPR026294; Makorin_3.
 IPR000571; Znf_CCCH.
 IPR001841; Znf_RING.
 IPR013083; Znf_RING/FYVE/PHD.
 IPR017907; Znf_RING_CS. 
Pfam
 PF13639; zf-RING_2 
SMART
 SM00184; RING
 SM00356; ZnF_C3H1 
PROSITE
 PS50103; ZF_C3H1
 PS00518; ZF_RING_1
 PS50089; ZF_RING_2 
PRINTS