CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018271
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Nuclear pore complex protein Nup133 
Protein Synonyms/Alias
 133 kDa nucleoporin; Nucleoporin Nup133 
Gene Name
 NUP133 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
35TPRTASRKGLPLGSAubiquitination[1, 2, 3, 4, 5]
82ESVNYDVKTFGSSLPubiquitination[4]
135IALSPITKLSVCKELubiquitination[3, 4]
239LIPESSGKIHQHILPubiquitination[4]
296LTSSNISKWELDDSSubiquitination[4, 6]
305ELDDSSEKHAYSWDIubiquitination[4, 6]
317WDINRALKENITDAIubiquitination[4, 6]
335ESNYEAIKEGVNIRYubiquitination[6]
518ETIAQEDKIKLLKAAubiquitination[4]
523EDKIKLLKAAFLQYCubiquitination[4]
604ILHQLEDKMKAHSFLubiquitination[4]
606HQLEDKMKAHSFLMDubiquitination[4]
674AILIALNKREYEIPSubiquitination[1, 4, 5, 6, 7]
758RREESLEKEPEYVPWubiquitination[4, 6]
787RQHEIVLKVAYPQADubiquitination[1, 4, 5, 6]
842LEMEYLQKRSDLLSPubiquitination[1, 2, 3, 4, 5, 6, 7]
908LFRWYLEKGKRGKLLubiquitination[4]
913LEKGKRGKLLSQPISubiquitination[4]
967ETRYFAKKKTLLGLSubiquitination[4]
968TRYFAKKKTLLGLSKubiquitination[4]
991SEDMLQEKIEEMAEQubiquitination[6]
1049RANEYDFKKALDLLEubiquitination[4, 6]
1089NWSSSDGKDDPIEVSubiquitination[6]
1097DDPIEVSKDSIFVKIubiquitination[4]
1107IFVKILQKLLKDGIQubiquitination[4]
1110KILQKLLKDGIQLSEubiquitination[1, 3, 4, 5, 6, 7]
1123SEYLPEVKDLLQADQubiquitination[7]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [6] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [7] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 
Functional Description
 Involved in poly(A)+ RNA transport. 
Sequence Annotation
 MOD_RES 1 1 N-acetylmethionine.
 MOD_RES 7 7 Phosphoserine.
 MOD_RES 27 27 Phosphoserine.
 MOD_RES 28 28 Phosphothreonine.
 MOD_RES 41 41 Phosphoserine.
 MOD_RES 45 45 Phosphoserine.
 MOD_RES 50 50 Phosphoserine.
 MOD_RES 480 480 Phosphoserine.
 MOD_RES 501 501 Phosphoserine (By similarity).  
Keyword
 3D-structure; Acetylation; Centromere; Chromosome; Complete proteome; Kinetochore; mRNA transport; Nuclear pore complex; Nucleus; Phosphoprotein; Polymorphism; Protein transport; Reference proteome; Translocation; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1156 AA 
Protein Sequence
MFPAAPSPRT PGTGSRRGPL AGLGPGSTPR TASRKGLPLG SAVSSPVLFS PVGRRSSLSS 60
RGTPTRMFPH HSITESVNYD VKTFGSSLPV KVMEALTLAE VDDQLTINID EGGWACLVCK 120
EKLIIWKIAL SPITKLSVCK ELQLPPSDFH WSADLVALSY SSPSGEAHST QAVAVMVATR 180
EGSIRYWPSL AGEDTYTEAF VDSGGDKTYS FLTAVQGGSF ILSSSGSQLI RLIPESSGKI 240
HQHILPQGQG MLSGIGRKVS SLFGILSPSS DLTLSSVLWD RERSSFYSLT SSNISKWELD 300
DSSEKHAYSW DINRALKENI TDAIWGSESN YEAIKEGVNI RYLDLKQNCD GLVILAAAWH 360
SADNPCLIYY SLITIEDNGC QMSDAVTVEV TQYNPPFQSE DLILCQLTVP NFSNQTAYLY 420
NESAVYVCST GTGKFSLPQE KIVFNAQGDS VLGAGACGGV PIIFSRNSGL VSITSRENVS 480
ILAEDLEGSL ASSVAGPNSE SMIFETTTKN ETIAQEDKIK LLKAAFLQYC RKDLGHAQMV 540
VDELFSSHSD LDSDSELDRA VTQISVDLMD DYPASDPRWA ESVPEEAPGF SNTSLIILHQ 600
LEDKMKAHSF LMDFIHQVGL FGRLGSFPVR GTPMATRLLL CEHAEKLSAA IVLKNHHSRL 660
SDLVNTAILI ALNKREYEIP SNLTPADVFF REVSQVDTIC ECLLEHEEQV LRDAPMDSIE 720
WAEVVINVNN ILKDMLQAAS HYRQNRNSLY RREESLEKEP EYVPWTATSG PGGIRTVIIR 780
QHEIVLKVAY PQADSNLRNI VTEQLVALID CFLDGYVSQL KSVDKSSNRE RYDNLEMEYL 840
QKRSDLLSPL LSLGQYLWAA SLAEKYCDFD ILVQMCEQTD NQSRLQRYMT QFADQNFSDF 900
LFRWYLEKGK RGKLLSQPIS QHGQLANFLQ AHEHLSWLHE INSQELEKAH ATLLGLANME 960
TRYFAKKKTL LGLSKLAALA SDFSEDMLQE KIEEMAEQER FLLHQETLPE QLLAEKQLNL 1020
SAMPVLTAPQ LIGLYICEEN RRANEYDFKK ALDLLEYIDE EEDININDLK LEILCKALQR 1080
DNWSSSDGKD DPIEVSKDSI FVKILQKLLK DGIQLSEYLP EVKDLLQADQ LGSLKSNPYF 1140
EFVLKANYEY YVQGQI 1156 
Gene Ontology
 GO:0000777; C:condensed chromosome kinetochore; IEA:UniProtKB-SubCell.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0031080; C:nuclear pore outer ring; IDA:UniProtKB.
 GO:0005487; F:nucleocytoplasmic transporter activity; IDA:UniProtKB.
 GO:0005975; P:carbohydrate metabolic process; TAS:Reactome.
 GO:0019221; P:cytokine-mediated signaling pathway; TAS:Reactome.
 GO:0015758; P:glucose transport; TAS:Reactome.
 GO:0000278; P:mitotic cell cycle; TAS:Reactome.
 GO:0006406; P:mRNA export from nucleus; IDA:UniProtKB.
 GO:0006999; P:nuclear pore organization; IMP:UniProtKB.
 GO:0048339; P:paraxial mesoderm development; IEA:Compara.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW.
 GO:0010827; P:regulation of glucose transport; TAS:Reactome.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome.
 GO:0055085; P:transmembrane transport; TAS:Reactome.
 GO:0022415; P:viral reproductive process; TAS:Reactome. 
Interpro
 IPR007187; Nucleoporin_Nup133/Nup155_C.
 IPR014908; Nucleoporin_Nup133/Nup155_N.
 IPR015943; WD40/YVTN_repeat-like_dom. 
Pfam
 PF03177; Nucleoporin_C
 PF08801; Nucleoporin_N 
SMART
  
PROSITE
  
PRINTS