CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-010265
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Lipid kinase YegS 
Protein Synonyms/Alias
  
Gene Name
 yegS 
Gene Synonyms/Alias
 b2086; JW2070 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
43HVRVTWEKGDAARYVacetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 
Functional Description
 In vitro phosphorylates phosphatidylglycerol but not diacylglycerol; the in vivo substrate is unknown. 
Sequence Annotation
 DOMAIN 2 133 DAGKc.
 NP_BIND 66 72 ATP (By similarity).
 ACT_SITE 271 271 Proton acceptor (By similarity).
 METAL 215 215 Magnesium; via carbonyl oxygen.
 METAL 218 218 Magnesium.
 METAL 220 220 Magnesium; via carbonyl oxygen.
 BINDING 40 40 ATP (By similarity).
 BINDING 95 95 ATP (By similarity).  
Keyword
 3D-structure; ATP-binding; Complete proteome; Cytoplasm; Kinase; Lipid biosynthesis; Lipid metabolism; Magnesium; Metal-binding; Nucleotide-binding; Phospholipid biosynthesis; Phospholipid metabolism; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 299 AA 
Protein Sequence
MAEFPASLLI LNGKSTDNLP LREAIMLLRE EGMTIHVRVT WEKGDAARYV EEARKFGVAT 60
VIAGGGDGTI NEVSTALIQC EGDDIPALGI LPLGTANDFA TSVGIPEALD KALKLAIAGD 120
AIAIDMAQVN KQTCFINMAT GGFGTRITTE TPEKLKAALG SVSYIIHGLM RMDTLQPDRC 180
EIRGENFHWQ GDALVIGIGN GRQAGGGQQL CPNALINDGL LQLRIFTGDE ILPALVSTLK 240
SDEDNPNIIE GASSWFDIQA PHDITFNLDG EPLSGQNFHI EILPAALRCR LPPDCPLLR 299 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:HAMAP.
 GO:0004143; F:diacylglycerol kinase activity; IEA:InterPro.
 GO:0001727; F:lipid kinase activity; IDA:EcoCyc.
 GO:0000287; F:magnesium ion binding; IEA:HAMAP.
 GO:0008654; P:phospholipid biosynthetic process; IEA:HAMAP.
 GO:0007205; P:protein kinase C-activating G-protein coupled receptor signaling pathway; IEA:InterPro. 
Interpro
 IPR005218; Diacylglycerol/lipid_kinase.
 IPR001206; Diacylglycerol_kinase_cat_dom.
 IPR022433; Lip_kinase_YegS.
 IPR022862; Lipid_kinase_YegS. 
Pfam
 PF00781; DAGK_cat 
SMART
 SM00046; DAGKc 
PROSITE
 PS50146; DAGK 
PRINTS