CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-015058
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Alpha-ketoglutarate-dependent dioxygenase alkB homolog 2 
Protein Synonyms/Alias
 Alkylated DNA repair protein alkB homolog 2; DNA oxidative demethylase ALKBH2; Oxy DC1 
Gene Name
 ALKBH2 
Gene Synonyms/Alias
 ABH2 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
7*MDRFLVKGAQGGLLubiquitination[1, 2]
16AQGGLLRKQEEQEPTubiquitination[2]
34PAVLGGDKESTRKRPubiquitination[2]
104ARVQVFGKWHSVPRKubiquitination[2, 3, 4]
162FVLINRYKDGCDHIGubiquitination[2]
Reference
 [1] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Dioxygenase that repairs alkylated DNA and RNA containing 1-methyladenine and 3-methylcytosine by oxidative demethylation. Can also repair alkylated DNA containing 1- ethenoadenine (in vitro). Has strong preference for double- stranded DNA. Has low efficiency with single-stranded substrates. Requires molecular oxygen, alpha-ketoglutarate and iron. 
Sequence Annotation
 DOMAIN 152 257 Fe2OG dioxygenase.
 REGION 102 104 Substrate binding.
 REGION 122 124 Substrate binding.
 REGION 159 161 Alpha-ketoglutarate binding.
 REGION 248 254 Alpha-ketoglutarate binding.
 METAL 171 171 Iron; catalytic.
 METAL 173 173 Iron; catalytic.
 METAL 236 236 Iron; catalytic.
 BINDING 174 174 Substrate.  
Keyword
 3D-structure; Alternative splicing; Complete proteome; Dioxygenase; DNA damage; DNA repair; Iron; Magnesium; Metal-binding; Nucleus; Oxidoreductase; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 261 AA 
Protein Sequence
MDRFLVKGAQ GGLLRKQEEQ EPTGEEPAVL GGDKESTRKR PRREAPGNGG HSAGPSWRHI 60
RAEGLDCSYT VLFGKAEADE IFQELEKEVE YFTGALARVQ VFGKWHSVPR KQATYGDAGL 120
TYTFSGLTLS PKPWIPVLER IRDHVSGVTG QTFNFVLINR YKDGCDHIGE HRDDERELAP 180
GSPIASVSFG ACRDFVFRHK DSRGKSPSRR VAVVRLPLAH GSLLMMNHPT NTHWYHSLPV 240
RKKVLAPRVN LTFRKILLTK K 261 
Gene Ontology
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0051747; F:cytosine C-5 DNA demethylase activity; IDA:UniProtKB.
 GO:0043734; F:DNA-N1-methyladenine dioxygenase activity; IDA:UniProtKB.
 GO:0008198; F:ferrous iron binding; IDA:UniProtKB.
 GO:0016702; F:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen; IEA:UniProtKB-KW.
 GO:0006307; P:DNA dealkylation involved in DNA repair; IDA:UniProtKB.
 GO:0035511; P:oxidative DNA demethylation; IDA:UniProtKB. 
Interpro
 IPR027450; AlkB-like.
 IPR005123; Oxoglu/Fe-dep_dioxygenase. 
Pfam
 PF13532; 2OG-FeII_Oxy_2 
SMART
  
PROSITE
 PS51471; FE2OG_OXY 
PRINTS