CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-030777
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 DNA polymerase eta 
Protein Synonyms/Alias
 cDNA FLJ52108, highly similar to DNA polymerase eta (EC 2.7.7.7) 
Gene Name
 POLH 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
29RGKANLTKYREASVEubiquitination[1]
69AVQERLQKLQGQPISubiquitination[2]
162SHNKVLAKLACGLNKubiquitination[1]
169KLACGLNKPNRQTLVubiquitination[1]
249GIEHDPVKPRQLPKTubiquitination[1]
400PVTSSEAKTQGSGPAubiquitination[1, 2]
424SLESFFQKAAERQKVubiquitination[1]
620SAVSHQGKRNPKSPLubiquitination[1, 2, 3, 4, 5, 6]
624HQGKRNPKSPLACTNubiquitination[1, 6]
647QTLESFFKPLTH***ubiquitination[4]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 651 AA 
Protein Sequence
MWADDAKKLC PDLLLAQVRE SRGKANLTKY REASVEVMEI MSRFAVIERA SIDEAYVDLT 60
SAVQERLQKL QGQPISADLL PSTYIEGLPQ GPTTAEETVQ KEGMRKQGLF QWLDSLQIDN 120
LTSPDLQLTV GAVIVEEMRA AIERETGFQC SAGISHNKVL AKLACGLNKP NRQTLVSHGS 180
VPQLFSQMPI RKIRSLGGKL GASVIEILGI EYMGELTQFT ESQLQSHFGE KNGSWLYAMC 240
RGIEHDPVKP RQLPKTIGCS KNFPGKTALA TREQVQWWLL QLAQELEERL TKDRNDNDRV 300
ATQLVVSIRV QGDKRLSSLR RCCALTRYDA HKMSHDAFTV IKNCNTSGIQ TEWSPPLTML 360
FLCATKFSAS APSSSTDITS FLSSDPSSLP KVPVTSSEAK TQGSGPAVTA TKKATTSLES 420
FFQKAAERQK VKEASLSSLT APTQAPMSNS PSKPSLPFQT SQSTGTEPFF KQKSLLLKQK 480
QLNNSSVSSP QQNPWSNCKA LPNSLPTEYP GCVPVCEGVS KLEESSKATP AEMDLAHNSQ 540
SMHASSASKS VLEVTQKATP NPSLLAAEDQ VPCEKCGSLV PVWDMPEHMD YHFALELQKS 600
FLQPHSSNPQ VVSAVSHQGK RNPKSPLACT NKRPRPEGMQ TLESFFKPLT H 651 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0003684; F:damaged DNA binding; IEA:InterPro.
 GO:0003887; F:DNA-directed DNA polymerase activity; IEA:Compara.
 GO:0006301; P:postreplication repair; IEA:Compara.
 GO:0006290; P:pyrimidine dimer repair; IEA:Compara. 
Interpro
 IPR017061; DNA_pol_eta.
 IPR017961; DNA_pol_Y-fam_little_finger.
 IPR001126; DNA_repair_prot_UmuC-like.
 IPR017963; DNA_repair_prot_UmuC-like_N. 
Pfam
 PF00817; IMS
 PF11799; IMS_C 
SMART
  
PROSITE
 PS50173; UMUC 
PRINTS