| Tag | Content |
|---|
| CPLM ID | CPLM-003150 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | DNA polymerase III subunit beta |
Protein Synonyms/Alias | Beta sliding clamp; Beta clamp |
Gene Name | dnaN |
Gene Synonyms/Alias | b3701; JW3678 |
Created Date | July 27, 2013 |
Organism | Escherichia coli (strain K12) |
NCBI Taxa ID | 83333 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 235 | GDFIFTSKLVDGRFP | acetylation | [1] | | 254 | VLPKNPDKHLEAGCD | acetylation | [1] | | 264 | EAGCDLLKQAFARAA | acetylation | [1] | | 277 | AAILSNEKFRGVRLY | acetylation | [1] |
|
Reference | [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli. Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C. Mol Cell. 2013 Jul 25;51(2):265-72. [ PMID: 23830618] |
Functional Description | DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication; once it is clamped onto DNA it slides freely (bidirectional and ATP- independent) along duplex DNA. Coordinates protein traffic at the replication fork, where it interacts with multiple DNA polymerases. |
Sequence Annotation | REGION 1 125 I.
REGION 126 253 II.
REGION 254 366 III. |
Keyword | 3D-structure; Complete proteome; Cytoplasm; DNA replication; DNA-directed DNA polymerase; Nucleotidyltransferase; Reference proteome; Transferase. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 366 AA |
Protein Sequence | MKFTVEREHL LKPLQQVSGP LGGRPTLPIL GNLLLQVADG TLSLTGTDLE MEMVARVALV 60
QPHEPGATTV PARKFFDICR GLPEGAEIAV QLEGERMLVR SGRSRFSLST LPAADFPNLD 120
DWQSEVEFTL PQATMKRLIE ATQFSMAHQD VRYYLNGMLF ETEGEELRTV ATDGHRLAVC 180
SMPIGQSLPS HSVIVPRKGV IELMRMLDGG DNPLRVQIGS NNIRAHVGDF IFTSKLVDGR 240
FPDYRRVLPK NPDKHLEAGC DLLKQAFARA AILSNEKFRG VRLYVSENQL KITANNPEQE 300
EAEEILDVTY SGAEMEIGFN VSYVLDVLNA LKCENVRMML TDSVSSVQIE DAASQSAAYV 360
VMPMRL 366 |
Gene Ontology | GO:0005829; C:cytosol; IDA:UniProtKB. GO:0009360; C:DNA polymerase III complex; IEA:InterPro. GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro. GO:0003677; F:DNA binding; IEA:InterPro. GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW. GO:0006271; P:DNA strand elongation involved in DNA replication; IDA:EcoCyc. GO:0006974; P:response to DNA damage stimulus; IEP:EcoliWiki. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |