CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-011387
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Recombining binding protein suppressor of hairless 
Protein Synonyms/Alias
 CBF-1; J kappa-recombination signal-binding protein; RBP-J kappa; RBP-J; RBP-JK; Renal carcinoma antigen NY-REN-30 
Gene Name
 RBPJ 
Gene Synonyms/Alias
 IGKJRB; IGKJRB1; RBPJK; RBPSUH 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
135LYISDSDKRKHFMLSubiquitination[1]
266LPRLIIRKVDKQTALacetylation[2]
291HKCAFYLKDTERMYLubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786] 
Functional Description
 Transcriptional regulator that plays a central role in Notch signaling, a signaling pathway involved in cell-cell communication that regulates a broad spectrum of cell-fate determinations. Acts as a transcriptional repressor when it is not associated with Notch proteins. When associated with some NICD product of Notch proteins (Notch intracellular domain), it acts as a transcriptional activator that activates transcription of Notch target genes. Probably represses or activates transcription via the recruitment of chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins, respectively. Specifically binds to the immunoglobulin kappa-type J segment recombination signal sequence. Binds specifically to methylated DNA. 
Sequence Annotation
 DOMAIN 355 445 IPT/TIG.
 DNA_BIND 58 65
 DNA_BIND 192 201
 DNA_BIND 265 297  
Keyword
 3D-structure; Activator; Alternative splicing; Complete proteome; Cytoplasm; Disease mutation; DNA-binding; Notch signaling pathway; Nucleus; Polymorphism; Reference proteome; Repeat; Repressor; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 500 AA 
Protein Sequence
MDHTEGSPAE EPPAHAPSPG KFGERPPPKR LTREAMRNYL KERGDQTVLI LHAKVAQKSY 60
GNEKRFFCPP PCVYLMGSGW KKKKEQMERD GCSEQESQPC AFIGIGNSDQ EMQQLNLEGK 120
NYCTAKTLYI SDSDKRKHFM LSVKMFYGNS DDIGVFLSKR IKVISKPSKK KQSLKNADLC 180
IASGTKVALF NRLRSQTVST RYLHVEGGNF HASSQQWGAF FIHLLDDDES EGEEFTVRDG 240
YIHYGQTVKL VCSVTGMALP RLIIRKVDKQ TALLDADDPV SQLHKCAFYL KDTERMYLCL 300
SQERIIQFQA TPCPKEPNKE MINDGASWTI ISTDKAEYTF YEGMGPVLAP VTPVPVVESL 360
QLNGGGDVAM LELTGQNFTP NLRVWFGDVE AETMYRCGES MLCVVPDISA FREGWRWVRQ 420
PVQVPVTLVR NDGIIYSTSL TFTYTPEPGP RPHCSAAGAI LRANSSQVPP NESNTNSEGS 480
YTNASTNSTS VTSSTATVVS 500 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:UniProtKB.
 GO:0002193; C:MAML1-RBP-Jkappa- ICN1 complex; IDA:UniProtKB.
 GO:0005730; C:nucleolus; IDA:UniProtKB.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0003677; F:DNA binding; TAS:UniProtKB.
 GO:0000150; F:recombinase activity; NAS:UniProtKB.
 GO:0000978; F:RNA polymerase II core promoter proximal region sequence-specific DNA binding; IEA:InterPro.
 GO:0001077; F:RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription; IDA:MGI.
 GO:0001525; P:angiogenesis; ISS:BHF-UCL.
 GO:0036302; P:atrioventricular canal development; ISS:BHF-UCL.
 GO:0097101; P:blood vessel endothelial cell fate specification; ISS:BHF-UCL.
 GO:0072554; P:blood vessel lumenization; ISS:BHF-UCL.
 GO:0003214; P:cardiac left ventricle morphogenesis; ISS:BHF-UCL.
 GO:0035912; P:dorsal aorta morphogenesis; ISS:BHF-UCL.
 GO:0003160; P:endocardium morphogenesis; ISS:BHF-UCL.
 GO:0003198; P:epithelial to mesenchymal transition involved in endocardial cushion formation; ISS:BHF-UCL.
 GO:0060716; P:labyrinthine layer blood vessel development; ISS:BHF-UCL.
 GO:0030279; P:negative regulation of ossification; ISS:BHF-UCL.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IMP:BHF-UCL.
 GO:0061314; P:Notch signaling involved in heart development; IC:BHF-UCL.
 GO:0003151; P:outflow tract morphogenesis; ISS:BHF-UCL.
 GO:0030513; P:positive regulation of BMP signaling pathway; ISS:BHF-UCL.
 GO:0060045; P:positive regulation of cardiac muscle cell proliferation; ISS:BHF-UCL.
 GO:2000138; P:positive regulation of cell proliferation involved in heart morphogenesis; ISS:BHF-UCL.
 GO:1901189; P:positive regulation of ephrin receptor signaling pathway; ISS:BHF-UCL.
 GO:1901186; P:positive regulation of ERBB signaling pathway; ISS:BHF-UCL.
 GO:0007221; P:positive regulation of transcription of Notch receptor target; IDA:UniProtKB.
 GO:0003256; P:regulation of transcription from RNA polymerase II promoter involved in myocardial precursor cell differentiation; ISS:BHF-UCL.
 GO:0006367; P:transcription initiation from RNA polymerase II promoter; TAS:Reactome.
 GO:0003222; P:ventricular trabecula myocardium morphogenesis; ISS:BHF-UCL. 
Interpro
 IPR015350; Beta-trefoil_DNA-bd_dom.
 IPR013783; Ig-like_fold.
 IPR014756; Ig_E-set.
 IPR002909; IPT_TIG_rcpt.
 IPR015351; LAG1_DNA-bd.
 IPR008967; p53-like_TF_DNA-bd. 
Pfam
 PF09270; BTD
 PF09271; LAG1-DNAbind
 PF01833; TIG 
SMART
  
PROSITE
  
PRINTS