CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-011768
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Tryptophan--tRNA ligase, cytoplasmic 
Protein Synonyms/Alias
 Tryptophanyl-tRNA synthetase; TrpRS 
Gene Name
 WRS1 
Gene Synonyms/Alias
 YOL097C; HRE432 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
18TQQVSELKSTDVKEQubiquitination[1]
49AQNIDYDKLIKQFGTacetylation[2]
101LDLYEQGKPFFLYTGacetylation[2]
153EKFLFKHKLTINDVKacetylation[2]
160KLTINDVKNFARENAubiquitination[1]
277ADKLKYSKPALLHSRacetylation[2]
320QIQKKINKYAFSGGQacetylation[2]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 
Functional Description
  
Sequence Annotation
 MOTIF 111 120 "HIGH" region.
 MOTIF 295 299 "KMSKS" region.  
Keyword
 3D-structure; Aminoacyl-tRNA synthetase; ATP-binding; Complete proteome; Cytoplasm; Ligase; Nucleotide-binding; Protein biosynthesis; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 432 AA 
Protein Sequence
MSNDETVEKV TQQVSELKST DVKEQVVTPW DVEGGVDEQG RAQNIDYDKL IKQFGTKPVN 60
EETLKRFKQV TGREPHHFLR KGLFFSERDF TKILDLYEQG KPFFLYTGRG PSSDSMHLGH 120
MIPFVFTKWL QEVFDVPLVI ELTDDEKFLF KHKLTINDVK NFARENAKDI IAVGFDPKNT 180
FIFSDLQYMG GAFYETVVRV SRQITGSTAK AVFGFNDSDC IGKFHFASIQ IATAFPSSFP 240
NVLGLPDKTP CLIPCAIDQD PYFRVCRDVA DKLKYSKPAL LHSRFFPALQ GSTTKMSASD 300
DTTAIFMTDT PKQIQKKINK YAFSGGQVSA DLHRELGGNP DVDVAYQYLS FFKDDDVFLK 360
ECYDKYKSGE LLSGEMKKLC IETLQEFVKA FQERRAQVDE ETLDKFMVPH KLVWGEKERL 420
VAPKPKTKQE KK 432 
Gene Ontology
 GO:0005737; C:cytoplasm; TAS:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004830; F:tryptophan-tRNA ligase activity; IDA:SGD.
 GO:0006436; P:tryptophanyl-tRNA aminoacylation; TAS:SGD. 
Interpro
 IPR001412; aa-tRNA-synth_I_CS.
 IPR002305; aa-tRNA-synth_Ic.
 IPR014729; Rossmann-like_a/b/a_fold.
 IPR002306; Trp-tRNA-ligase. 
Pfam
 PF00579; tRNA-synt_1b 
SMART
  
PROSITE
 PS00178; AA_TRNA_LIGASE_I 
PRINTS
 PR01039; TRNASYNTHTRP.