CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-021381
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Sentrin-specific protease 3 
Protein Synonyms/Alias
 SUMO-1-specific protease 3; Sentrin/SUMO-specific protease SENP3 
Gene Name
 SENP3 
Gene Synonyms/Alias
 SSP3; SUSP3 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
43PPPKPRLKSGGGFGPubiquitination[1, 2, 3, 4]
231DGLRWTPKSPLDPDSubiquitination[3]
357EFSTPSRKGLVLQLIubiquitination[3]
446DGVKRWTKNVDIFNKubiquitination[1, 3, 4]
497RCPKHIAKYLQAEAVubiquitination[3]
505YLQAEAVKKDRLDFHubiquitination[3]
506LQAEAVKKDRLDFHQubiquitination[3]
516LDFHQGWKGYFKMNVubiquitination[3]
520QGWKGYFKMNVARQNubiquitination[3]
541AFVLQYCKHLALSQPubiquitination[3]
558FTQQDMPKLRRQIYKubiquitination[3]
565KLRRQIYKELCHCKLubiquitination[3]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Protease that releases SUMO2 and SUMO3 monomers from sumoylated substrates, but has only weak activity against SUMO1 conjugates. Deconjugates SUMO2 from MEF2D, which increases its transcriptional activation capability. Deconjugates SUMO2 and SUMO3 from CDCA8. Redox sensor that, when redistributed into nucleoplasm, can act as an effector to enhance HIF1A transcriptional activity by desumoylating EP300. Required for rRNA processing through deconjugation of SUMO2 and SUMO3 from nucleophosmin, NPM1. Plays a role in the regulation of sumoylation status of ZNF148. Functions as a component of the Five Friends of Methylated CHTOP (5FMC) complex; the 5FMC complex is recruited to ZNF148 by methylated CHTOP, leading to desumoylation of ZNF148 and subsequent transactivation of ZNF148 target genes. 
Sequence Annotation
 REGION 386 543 Protease.
 MOTIF 125 128 Nuclear localization signal (Potential).
 MOTIF 153 159 Nuclear localization signal (Potential).
 ACT_SITE 465 465 By similarity.
 ACT_SITE 482 482 By similarity.
 ACT_SITE 532 532 By similarity.
 MOD_RES 169 169 Phosphoserine.
 MOD_RES 181 181 Phosphoserine.
 MOD_RES 212 212 Phosphoserine.
 MOD_RES 232 232 Phosphoserine.
 MOD_RES 353 353 Phosphothreonine.  
Keyword
 Complete proteome; Cytoplasm; Hydrolase; Nucleus; Phosphoprotein; Polymorphism; Protease; Reference proteome; Thiol protease; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 574 AA 
Protein Sequence
MKETIQGTGS WGPEPPGPGI PPAYSSPRRE RLRWPPPPKP RLKSGGGFGP DPGSGTTVPA 60
RRLPVPRPSF DASASEEEEE EEEEEDEDEE EEVAAWRLPP RWSQLGTSQR PRPSRPTHRK 120
TCSQRRRRAM RAFRMLLYSK STSLTFHWKL WGRHRGRRRG LAHPKNHLSP QQGGATPQVP 180
SPCCRFDSPR GPPPPRLGLL GALMAEDGVR GSPPVPSGPP MEEDGLRWTP KSPLDPDSGL 240
LSCTLPNGFG GQSGPEGERS LAPPDASILI SNVCSIGDHV AQELFQGSDL GMAEEAERPG 300
EKAGQHSPLR EEHVTCVQSI LDEFLQTYGS LIPLSTDEVV EKLEDIFQQE FSTPSRKGLV 360
LQLIQSYQRM PGNAMVRGFR VAYKRHVLTM DDLGTLYGQN WLNDQVMNMY GDLVMDTVPE 420
KVHFFNSFFY DKLRTKGYDG VKRWTKNVDI FNKELLLIPI HLEVHWSLIS VDVRRRTITY 480
FDSQRTLNRR CPKHIAKYLQ AEAVKKDRLD FHQGWKGYFK MNVARQNNDS DCGAFVLQYC 540
KHLALSQPFS FTQQDMPKLR RQIYKELCHC KLTV 574 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0071339; C:MLL1 complex; IDA:UniProtKB.
 GO:0005730; C:nucleolus; IDA:LIFEdb.
 GO:0004843; F:ubiquitin-specific protease activity; IEA:Compara.
 GO:0006508; P:proteolysis; IEA:UniProtKB-KW. 
Interpro
 IPR003653; Peptidase_C48. 
Pfam
 PF02902; Peptidase_C48 
SMART
  
PROSITE
 PS50600; ULP_PROTEASE 
PRINTS