CPLM-007162

Genbank Nucleotide ID

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit

Protein Synonyms/Alias

DDOST 48 kDa subunit; Oligosaccharyl transferase 48 kDa subunit

KIAA0115; OST48; OK/SW-cl.45

Homo sapiens (Human)

Position	Peptide	Type	References
77	RGFELTFKTADDPSL	ubiquitination	[1, 2]
182	ADTENLLKAPTIVGK	ubiquitination	[1, 2]
189	KAPTIVGKSSLNPIL	ubiquitination	[1, 2, 3, 4, 5, 6, 7]
301	ALSRWVFKEEGVLRV	ubiquitination	[1, 2, 3, 4, 5, 6, 7]
391	VYGVFQFKVDYNRLG	ubiquitination	[1]

[1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
[3] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
[4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[5] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
[6] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[7] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]

Functional Description

Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains.

Complete proteome; Congenital disorder of glycosylation; Disease mutation; Endoplasmic reticulum; Glycosyltransferase; Membrane; Polymorphism; Reference proteome; Signal; Transferase; Transmembrane; Transmembrane helix.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
GO:0008250; C:oligosaccharyltransferase complex; ISS:UniProtKB.
GO:0004579; F:dolichyl-diphosphooligosaccharide-protein glycotransferase activity; IEA:InterPro.
GO:0045087; P:innate immune response; TAS:Reactome.
GO:0043687; P:post-translational protein modification; TAS:Reactome.
GO:0018279; P:protein N-linked glycosylation via asparagine; IC:HGNC.
GO:0034097; P:response to cytokine stimulus; IDA:UniProtKB.
GO:0006614; P:SRP-dependent cotranslational protein targeting to membrane; TAS:Reactome.
GO:0042110; P:T cell activation; IDA:UniProtKB.
GO:0006412; P:translation; TAS:Reactome.