CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-021722
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Na(+)/H(+) exchange regulatory cofactor NHE-RF2 
Protein Synonyms/Alias
 NHERF-2; NHE3 kinase A regulatory protein E3KARP; Octs2; SRY-interacting protein 1; SIP-1; Sodium-hydrogen exchanger regulatory factor 2; Solute carrier family 9 isoform A3 regulatory factor 2; Tyrosine kinase activator protein 1; TKA-1 
Gene Name
 Slc9a3r2 
Gene Synonyms/Alias
 Nherf2 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
58GFNLHSDKSRPGQYIubiquitination[1]
126PETDEHFKRLRVVPTubiquitination[1]
171SDLPGSEKDNEDGSTubiquitination[1]
180NEDGSTWKRDPFQESubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023
Functional Description
 Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May also act as scaffold protein in the nucleus (By similarity). 
Sequence Annotation
 DOMAIN 11 91 PDZ 1.
 DOMAIN 151 231 PDZ 2.
 MOD_RES 183 183 Phosphoserine (By similarity).
 MOD_RES 254 254 Phosphoserine (By similarity).
 MOD_RES 272 272 Phosphoserine.  
Keyword
 Alternative splicing; Cell membrane; Complete proteome; Membrane; Nucleus; Phosphoprotein; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 337 AA 
Protein Sequence
MARSRTSMLP ASAPGAPPVN SQLGLTQDVN GPPRELRPRL CHLRRGPQGY GFNLHSDKSR 60
PGQYIRSVDP GSPASHSGLR AQDRLIEVNG QNVEGLRHAE VVARIKAQED EARLLVVDPE 120
TDEHFKRLRV VPTEEHVEGP LPSPVTNGTS PAQLNGGSVC SSRSDLPGSE KDNEDGSTWK 180
RDPFQESGLH LSPTAAEAKE KARATRVNKR APQMDWNRKR EIFSNF 226 
Gene Ontology
 GO:0016324; C:apical plasma membrane; IDA:MGI.
 GO:0005737; C:cytoplasm; IDA:MGI.
 GO:0012505; C:endomembrane system; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IDA:MGI.
 GO:0014067; P:negative regulation of phosphatidylinositol 3-kinase cascade; IGI:MGI. 
Interpro
 IPR015098; EBP50_C-term.
 IPR017300; NaH_exchngr_reg_CF_NHE-RF.
 IPR001478; PDZ. 
Pfam
 PF09007; EBP50_C-term
 PF00595; PDZ 
SMART
 SM00228; PDZ 
PROSITE
 PS50106; PDZ 
PRINTS