CPLM-000696

Genbank Nucleotide ID

Heterogeneous nuclear ribonucleoprotein R

Protein Synonyms/Alias

Homo sapiens (Human)

Position	Peptide	Type	References
41	IEAGLPQKVAERLDE	ubiquitination	[1, 2]
103	AFLCGVMKTYRQREK	acetylation	[3]
103	AFLCGVMKTYRQREK	ubiquitination	[4, 5]
114	QREKQGSKVQESTKG	ubiquitination	[5]
171	GTEVFVGKIPRDLYE	ubiquitination	[5, 6, 7]
187	ELVPLFEKAGPIWDL	ubiquitination	[6, 7, 8]
224	EAAQEAVKLCDSYEI	ubiquitination	[5, 9]
235	SYEIRPGKHLGVCIS	methylation	[10]
255	LFVGSIPKNKTKENI	ubiquitination	[1, 2]
257	VGSIPKNKTKENILE	ubiquitination	[5]
259	SIPKNKTKENILEEF	ubiquitination	[4]
300	FLEYEDHKSAAQARR	ubiquitination	[5]
341	PEVMAKVKVLFVRNL	ubiquitination	[1, 4, 5]
359	VTEEILEKSFSEFGK	ubiquitination	[2, 5, 6, 11]
366	KSFSEFGKLERVKKL	acetylation	[3]
366	KSFSEFGKLERVKKL	ubiquitination	[1, 2, 4, 5, 6]
374	LERVKKLKDYAFVHF	ubiquitination	[4, 5]
389	EDRGAAVKAMDEMNG	acetylation	[3]
389	EDRGAAVKAMDEMNG	ubiquitination	[4, 5, 6]
397	AMDEMNGKEIEGEEI	acetylation	[3]

[1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
[3] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
[4] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
[5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[6] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[7] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
[8] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
[9] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
[10] Large-scale global identification of protein lysine methylation in vivo.
Cao XJ, Arnaudo AM, Garcia BA.
Epigenetics. 2013 May 1;8(5):477-85. [PMID: 23644510]
[11] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]

Functional Description

Component of ribonucleosomes, which are complexes of at least 20 other different heterogenious nuclear ribonucleoproteins (hnRNP). hnRNP play an important role in processing of precursor mRNA in the nucleus.

DOMAIN 165 244 RRM 1.
DOMAIN 246 328 RRM 2.
DOMAIN 341 411 RRM 3.
REPEAT 462 471 1; approximate.
REPEAT 472 482 2.
REPEAT 488 497 3; approximate.
REGION 447 567 RNA-binding RGG-box.
REGION 462 497 3 X 11 AA approximate repeats of D-D-Y-Y-
MOTIF 412 418 Nuclear localization signal (Potential).
MOD_RES 2 2 N-acetylalanine.
MOD_RES 366 366 N6-acetyllysine.

3D-structure; Acetylation; Alternative splicing; Complete proteome; Cytoplasm; Direct protein sequencing; mRNA processing; mRNA splicing; Nucleus; Reference proteome; Repeat; Ribonucleoprotein; RNA-binding; Spliceosome.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0071013; C:catalytic step 2 spliceosome; IDA:UniProtKB.
GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
GO:0005654; C:nucleoplasm; IDA:UniProtKB.
GO:0000166; F:nucleotide binding; IEA:InterPro.
GO:0003723; F:RNA binding; NAS:UniProtKB.
GO:0000398; P:mRNA splicing, via spliceosome; IC:UniProtKB.

IPR006535; HnRNP_R/Q_splicing_fac.
IPR012677; Nucleotide-bd_a/b_plait.
IPR000504; RRM_dom.