CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022941
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Prenylcysteine oxidase 1 
Protein Synonyms/Alias
 Prenylcysteine lyase 
Gene Name
 PCYOX1 
Gene Synonyms/Alias
 KIAA0908; PCL1; UNQ597/PRO1183 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
162WVEDVLDKFMRIYRYubiquitination[1, 2]
205TLLETLQKAGFSEKFubiquitination[2, 3]
415FSQETLTKAQILKLFubiquitination[4]
500DQDGLYEKLKTEL**ubiquitination[2, 3]
Reference
 [1] Tryptic digestion of ubiquitin standards reveals an improved strategy for identifying ubiquitinated proteins by mass spectrometry.
 Denis NJ, Vasilescu J, Lambert JP, Smith JC, Figeys D.
 Proteomics. 2007 Mar;7(6):868-74. [PMID: 17370265]
 [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965] 
Functional Description
 Involved in the degradation of prenylated proteins. Cleaves the thioether bond of prenyl-L-cysteines, such as farnesylcysteine and geranylgeranylcysteine. 
Sequence Annotation
 CARBOHYD 196 196 N-linked (GlcNAc...).
 CARBOHYD 323 323 N-linked (GlcNAc...).
 CARBOHYD 353 353 N-linked (GlcNAc...).
 CROSSLNK 162 162 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 Complete proteome; Direct protein sequencing; FAD; Glycoprotein; Isopeptide bond; Lysosome; Oxidoreductase; Polymorphism; Reference proteome; Signal; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 505 AA 
Protein Sequence
MGRVVAELVS SLLGLWLLLC SCGCPEGAEL RAPPDKIAII GAGIGGTSAA YYLRQKFGKD 60
VKIDLFEREE VGGRLATMMV QGQEYEAGGS VIHPLNLHMK RFVKDLGLSA VQASGGLLGI 120
YNGETLVFEE SNWFIINVIK LVWRYGFQSL RMHMWVEDVL DKFMRIYRYQ SHDYAFSSVE 180
KLLHALGGDD FLGMLNRTLL ETLQKAGFSE KFLNEMIAPV MRVNYGQSTD INAFVGAVSL 240
SCSDSGLWAV EGGNKLVCSG LLQASKSNLI SGSVMYIEEK TKTKYTGNPT KMYEVVYQIG 300
TETRSDFYDI VLVATPLNRK MSNITFLNFD PPIEEFHQYY QHIVTTLVKG ELNTSIFSSR 360
PIDKFGLNTV LTTDNSDLFI NSIGIVPSVR EKEDPEPSTD GTYVWKIFSQ ETLTKAQILK 420
LFLSYDYAVK KPWLAYPHYK PPEKCPSIIL HDRLYYLNGI ECAASAMEMS AIAAHNAALL 480
AYHRWNGHTD MIDQDGLYEK LKTEL 505 
Gene Ontology
 GO:0005764; C:lysosome; IDA:BHF-UCL.
 GO:0005886; C:plasma membrane; IEA:Compara.
 GO:0034361; C:very-low-density lipoprotein particle; IDA:BHF-UCL.
 GO:0008555; F:chloride-transporting ATPase activity; IEA:Compara.
 GO:0001735; F:prenylcysteine oxidase activity; IDA:BHF-UCL.
 GO:0030327; P:prenylated protein catabolic process; NAS:UniProtKB.
 GO:0030328; P:prenylcysteine catabolic process; IEA:Compara.
 GO:0030329; P:prenylcysteine metabolic process; NAS:UniProtKB. 
Interpro
 IPR010795; Prenylcys_lyase.
 IPR017046; Prenylcysteine_Oxase. 
Pfam
 PF07156; Prenylcys_lyase 
SMART
  
PROSITE
  
PRINTS