UniProt Accession

 RHEB_HUMAN; Q15382; B3KWN6; D3DX13; Q53Y56; Q99444 

Genbank Protein ID

 Z29677; D78132; AF148645; AF493921; AK125446; BT006958; AC005996; CH471173; BC016155; BC107705 

Genbank Nucleotide ID

 CAA82774.1; BAA11211.1; AAF73125.1; AAM12635.1; BAG54198.1; AAP35604.1; AAD15548.1; EAW53989.1; AAH16155.1; AAI07706.1 

Protein Name

 GTP-binding protein Rheb 

Protein Synonyms/Alias

 Ras homolog enriched in brain 

Gene Name

 RHEB 

Gene Synonyms/Alias

 RHEB2 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
120	PIMLVGNKKDLHMER	ubiquitination	[1, 2]
121	IMLVGNKKDLHMERV	ubiquitination	[3]
135	VISYEEGKALAESWN	ubiquitination	[1, 2, 3]
151	AFLESSAKENQTAVD	ubiquitination	[1, 2, 3]
169	RIILEAEKMDGAASQ	ubiquitination	[1, 3, 4]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473] 

Functional Description

 Stimulates the phosphorylation of S6K1 and EIF4EBP1 through activation of mTORC1 signaling. Activates the protein kinase activity of mTORC1. Has low intrinsic GTPase activity. 

Sequence Annotation

 NP_BIND 16 21 GTP.
 NP_BIND 32 38 GTP.
 NP_BIND 119 122 GTP.
 NP_BIND 149 150 GTP.
 MOTIF 35 43 Effector region (By similarity).
 METAL 20 20 Magnesium.
 METAL 38 38 Magnesium.
 MOD_RES 130 130 Phosphoserine; by MAPKAPK5 (By
 MOD_RES 181 181 Cysteine methyl ester (Probable).
 LIPID 181 181 S-farnesyl cysteine.  

Keyword

 3D-structure; Cell membrane; Complete proteome; GTP-binding; Lipoprotein; Magnesium; Membrane; Metal-binding; Methylation; Nucleotide-binding; Phosphoprotein; Polymorphism; Prenylation; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 184 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0030425; C:dendrite; IEA:Compara.
 GO:0016020; C:membrane; TAS:ProtInc.
 GO:0043025; C:neuronal cell body; IEA:Compara.
 GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
 GO:0005681; C:spliceosomal complex; IEA:Compara.
 GO:0019003; F:GDP binding; IEA:Compara.
 GO:0005525; F:GTP binding; ISS:UniProtKB.
 GO:0003924; F:GTPase activity; TAS:ProtInc.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0007050; P:cell cycle arrest; TAS:Reactome.
 GO:0008286; P:insulin receptor signaling pathway; TAS:Reactome.
 GO:0032008; P:positive regulation of TOR signaling cascade; IMP:UniProtKB.
 GO:0007264; P:small GTPase mediated signal transduction; IEA:Compara. 

Interpro

 IPR027417; P-loop_NTPase.
 IPR005225; Small_GTP-bd_dom.
 IPR001806; Small_GTPase.
 IPR020849; Small_GTPase_Ras. 

Pfam

 PF00071; Ras 

SMART

 SM00173; RAS 

PROSITE

 PS51421; RAS 

PRINTS

 PR00449; RASTRNSFRMNG.