CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006177
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Heat shock protein homolog SSE1 
Protein Synonyms/Alias
 Chaperone protein MSI3 
Gene Name
 SSE1 
Gene Synonyms/Alias
 MSI3; YPL106C; LPG3C 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
86PDFEQESKHFTSKLVacetylation[1]
86PDFEQESKHFTSKLVubiquitination[2]
91ESKHFTSKLVELDDKacetylation[1]
98KLVELDDKKTGAEVRacetylation[1]
125LAAMFIDKVKDTVKQubiquitination[3]
195DLPEGEEKPRIVAFVacetylation[1]
195DLPEGEEKPRIVAFVubiquitination[2, 3]
222AFKKGQLKVLGTACDacetylation[1]
230VLGTACDKHFGGRDFacetylation[1]
230VLGTACDKHFGGRDFubiquitination[2]
273RILTAAEKLKKVLSAacetylation[1]
273RILTAAEKLKKVLSAubiquitination[2]
311EELEELVKPLLERVTacetylation[1]
323RVTEPVTKALAQAKLacetylation[1]
323RVTEPVTKALAQAKLubiquitination[2]
429GSSFPSTKLITLNRTubiquitination[2]
545HTFGLDAKKLNELIEacetylation[1]
553KLNELIEKENEMLAQacetylation[1]
562NEMLAQDKLVAETEDacetylation[1]
584YIYTLRGKLEEEYAPacetylation[1]
584YIYTLRGKLEEEYAPubiquitination[2]
598PFASDAEKTKLQGMLacetylation[1]
607KLQGMLNKAEEWLYDubiquitination[2]
621DEGFDSIKAKYIAKYacetylation[1]
627IKAKYIAKYEELASLacetylation[1]
645IRGRYLAKEEEKKQAacetylation[1]
656KKQAIRSKQEASQMAubiquitination[2]
668QMAAMAEKLAAQRKAacetylation[1]
668QMAAMAEKLAAQRKAubiquitination[2]
683EAEKKEEKKDTEGDVubiquitination[2]
684AEKKEEKKDTEGDVDubiquitination[2]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [3] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047] 
Functional Description
 Has a calcium-dependent calmodulin-binding activity. Required for normal growth at various temperatures. 
Sequence Annotation
 MOD_RES 2 2 N-acetylserine.
 MOD_RES 660 660 Phosphoserine.  
Keyword
 3D-structure; Acetylation; ATP-binding; Calmodulin-binding; Chaperone; Complete proteome; Cytoplasm; Direct protein sequencing; Nucleotide-binding; Phosphoprotein; Reference proteome; Stress response. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 693 AA 
Protein Sequence
MSTPFGLDLG NNNSVLAVAR NRGIDIVVNE VSNRSTPSVV GFGPKNRYLG ETGKNKQTSN 60
IKNTVANLKR IIGLDYHHPD FEQESKHFTS KLVELDDKKT GAEVRFAGEK HVFSATQLAA 120
MFIDKVKDTV KQDTKANITD VCIAVPPWYT EEQRYNIADA ARIAGLNPVR IVNDVTAAGV 180
SYGIFKTDLP EGEEKPRIVA FVDIGHSSYT CSIMAFKKGQ LKVLGTACDK HFGGRDFDLA 240
ITEHFADEFK TKYKIDIREN PKAYNRILTA AEKLKKVLSA NTNAPFSVES VMNDVDVSSQ 300
LSREELEELV KPLLERVTEP VTKALAQAKL SAEEVDFVEI IGGTTRIPTL KQSISEAFGK 360
PLSTTLNQDE AIAKGAAFIC AIHSPTLRVR PFKFEDIHPY SVSYSWDKQV EDEDHMEVFP 420
AGSSFPSTKL ITLNRTGDFS MAASYTDITQ LPPNTPEQIA NWEITGVQLP EGQDSVPVKL 480
KLRCDPSGLH TIEEAYTIED IEVEEPIPLP EDAPEDAEQE FKKVTKTVKK DDLTIVAHTF 540
GLDAKKLNEL IEKENEMLAQ DKLVAETEDR KNTLEEYIYT LRGKLEEEYA PFASDAEKTK 600
LQGMLNKAEE WLYDEGFDSI KAKYIAKYEE LASLGNIIRG RYLAKEEEKK QAIRSKQEAS 660
QMAAMAEKLA AQRKAEAEKK EEKKDTEGDV DMD 693 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0005844; C:polysome; IDA:SGD.
 GO:0000774; F:adenyl-nucleotide exchange factor activity; IDA:SGD.
 GO:0005524; F:ATP binding; IDA:SGD.
 GO:0042277; F:peptide binding; IDA:SGD.
 GO:0042026; P:protein refolding; IDA:SGD.
 GO:0006950; P:response to stress; IEA:UniProtKB-KW. 
Interpro
 IPR018181; Heat_shock_70_CS.
 IPR013126; Hsp_70_fam. 
Pfam
 PF00012; HSP70 
SMART
  
PROSITE
 PS00297; HSP70_1
 PS00329; HSP70_2
 PS01036; HSP70_3 
PRINTS
 PR00301; HEATSHOCK70.