UniProt Accession

 GNAI2_MOUSE; P08752; Q3TXK7; Q6P1C0 

Genbank Protein ID

 M13963; AK157998; AK159222; AK167388; BC065159; S71213 

Genbank Nucleotide ID

 AAA37692.1; BAE34308.1; BAE34909.1; BAE39478.1; AAH65159.1; AAB30632.2 

Protein Name

 Guanine nucleotide-binding protein G(i) subunit alpha-2 

Protein Synonyms/Alias

 Adenylate cyclase-inhibiting G alpha protein 

Gene Name

 Gnai2 

Gene Synonyms/Alias

 Gnai-2 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
29	NLREDGEKAAREVKL	ubiquitination	[1]
35	EKAAREVKLLLLGAG	ubiquitination	[1]
46	LGAGESGKSTIVKQM	ubiquitination	[1]
193	VETHFTFKDLHFKMF	ubiquitination	[1]
198	TFKDLHFKMFDVGGQ	ubiquitination	[1]
249	NRMHESMKLFDSICN	ubiquitination	[1]
272	IILFLNKKDLFEEKI	ubiquitination	[1]
296	PEYTGANKYDEAASY	ubiquitination	[1]
307	AASYIQSKFEDLNKR	ubiquitination	[1]
313	SKFEDLNKRKDTKEI	ubiquitination	[1]
350	VIIKNNLKDCGLF**	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. May play a role in cell division. 

Sequence Annotation

 NP_BIND 40 47 GTP (By similarity).
 NP_BIND 176 182 GTP (By similarity).
 NP_BIND 201 205 GTP (By similarity).
 NP_BIND 270 273 GTP (By similarity).
 METAL 47 47 Magnesium (By similarity).
 METAL 182 182 Magnesium (By similarity).
 BINDING 327 327 GTP; via amide nitrogen (By similarity).
 LIPID 2 2 N-myristoyl glycine (By similarity).
 LIPID 3 3 S-palmitoyl cysteine (By similarity).  

Keyword

 Cell cycle; Cell division; Cell membrane; Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing; GTP-binding; Lipoprotein; Magnesium; Membrane; Metal-binding; Myristate; Nucleotide-binding; Palmitate; Reference proteome; Transducer. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 355 AA 

Protein Sequence

Gene Ontology

 GO:0005813; C:centrosome; ISS:UniProtKB.
 GO:0005737; C:cytoplasm; ISS:UniProtKB.
 GO:0005829; C:cytosol; IEA:Compara.
 GO:0005834; C:heterotrimeric G-protein complex; IBA:RefGenome.
 GO:0045121; C:membrane raft; IEA:Compara.
 GO:0030496; C:midbody; ISS:UniProtKB.
 GO:0005634; C:nucleus; IEA:Compara.
 GO:0031683; F:G-protein beta/gamma-subunit complex binding; IBA:RefGenome.
 GO:0001664; F:G-protein coupled receptor binding; IBA:RefGenome.
 GO:0005525; F:GTP binding; ISA:MGI.
 GO:0003924; F:GTPase activity; IBA:RefGenome.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0004871; F:signal transducer activity; IBA:RefGenome.
 GO:0000186; P:activation of MAPKK activity; IEA:Compara.
 GO:0001973; P:adenosine receptor signaling pathway; IBA:RefGenome.
 GO:0007193; P:adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway; IMP:MGI.
 GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
 GO:0051301; P:cell division; ISS:UniProtKB.
 GO:0008283; P:cell proliferation; IPI:MGI.
 GO:0007213; P:G-protein coupled acetylcholine receptor signaling pathway; IMP:MGI.
 GO:0007214; P:gamma-aminobutyric acid signaling pathway; IBA:RefGenome.
 GO:0007243; P:intracellular protein kinase cascade; IEA:Compara.
 GO:0050805; P:negative regulation of synaptic transmission; IEA:Compara.
 GO:0008284; P:positive regulation of cell proliferation; IEA:Compara.
 GO:0051924; P:regulation of calcium ion transport; IEA:Compara. 

Interpro

 IPR001408; Gprotein_alpha_I.
 IPR001019; Gprotein_alpha_su.
 IPR011025; GproteinA_insert.
 IPR027417; P-loop_NTPase. 

Pfam

 PF00503; G-alpha 

SMART

 SM00275; G_alpha 

PROSITE

  

PRINTS

 PR00318; GPROTEINA.
 PR00441; GPROTEINAI.