UniProt Accession

 OFUT1_HUMAN; Q9H488; A8K4R8; E1P5M4; Q14685; Q5W185; Q9BW76 

Genbank Protein ID

 AF375884; D80002; AL121897; AL121897; AK291033; CH471077; CH471077; CH471077; BC000582 

Genbank Nucleotide ID

 AAL09576.1; BAA11497.2; CAC16424.1; CAH73300.1; BAF83722.1; EAW76383.1; EAW76384.1; EAW76385.1; AAH00582.1 

Protein Name

 GDP-fucose protein O-fucosyltransferase 1 

Protein Synonyms/Alias

 Peptide-O-fucosyltransferase 1; O-FucT-1 

Gene Name

 POFUT1 

Gene Synonyms/Alias

 FUT12; KIAA0180 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
92	VSYQKYFKLEPLQAY	ubiquitination	[1]
246	LRIGSDWKNACAMLK	ubiquitination	[1]
295	KEIQRAVKLWVRSLD	ubiquitination	[1]
324	PELQQLFKGKVKVVS	ubiquitination	[2]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 

Functional Description

 Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue found in the consensus sequence C2-X(4,5)-[S/T]-C3 of EGF domains, where C2 and C3 are the second and third conserved cysteines. Specifically uses GDP-fucose as donor substrate and proper disulfide pairing of the substrate EGF domains is required for fucose transfer. Plays a crucial role in NOTCH signaling. Initial fucosylation of NOTCH by POFUT1 generates a substrate for FRINGE/RFNG, an acetylglucosaminyltransferase that can then extend the fucosylation on the NOTCH EGF repeats. This extended fucosylation is required for optimal ligand binding and canonical NOTCH signaling induced by DLL1 or JAGGED1. Fucosylates AGRN and determines its ability to cluster acetylcholine receptors (AChRs). 

Sequence Annotation

 REGION 44 46 Substrate binding (By similarity).
 REGION 238 240 Substrate binding (By similarity).
 REGION 356 357 Substrate binding (By similarity).
 MOTIF 385 388 Prevents secretion from ER (Potential).
 BINDING 335 335 Substrate (By similarity).
 CARBOHYD 62 62 N-linked (GlcNAc...) (Potential).
 CARBOHYD 160 160 N-linked (GlcNAc...).
 DISULFID 38 40 By similarity.
 DISULFID 126 140 By similarity.
 DISULFID 249 283 By similarity.
 DISULFID 267 354 By similarity.  

Keyword

 Alternative splicing; Carbohydrate metabolism; Complete proteome; Disulfide bond; Endoplasmic reticulum; Fucose metabolism; Glycoprotein; Glycosyltransferase; Manganese; Notch signaling pathway; Polymorphism; Reference proteome; Signal; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 388 AA 

Protein Sequence

Gene Ontology

 GO:0005783; C:endoplasmic reticulum; IDA:UniProtKB.
 GO:0016020; C:membrane; IDA:UniProtKB.
 GO:0046922; F:peptide-O-fucosyltransferase activity; IDA:UniProtKB.
 GO:0001525; P:angiogenesis; IEA:Compara.
 GO:0009790; P:embryo development; NAS:UniProtKB.
 GO:0006004; P:fucose metabolic process; IEA:UniProtKB-KW.
 GO:0007507; P:heart development; IEA:Compara.
 GO:0007399; P:nervous system development; IEA:Compara.
 GO:0007219; P:Notch signaling pathway; NAS:UniProtKB.
 GO:0016266; P:O-glycan processing; TAS:UniProtKB.
 GO:0006355; P:regulation of transcription, DNA-dependent; NAS:UniProtKB.
 GO:0001756; P:somitogenesis; IEA:Compara. 

Interpro

 IPR019378; GDP-Fuc_O-FucTrfase. 

Pfam

 PF10250; O-FucT 

SMART

  

PROSITE

  

PRINTS