| Tag | Content |
|---|
| CPLM ID | CPLM-008720 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Coatomer subunit alpha |
Protein Synonyms/Alias | Alpha-coat protein; Alpha-COP; Retrieval from endoplasmic reticulum protein 1; Secretory protein 22; Suppressor of osmo-sensitivity 1 |
Gene Name | COP1 |
Gene Synonyms/Alias | RET1; SEC33; SOO1; YDL145C; D1578 |
Created Date | July 27, 2013 |
Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
NCBI Taxa ID | 559292 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 6 | **MKMLTKFESKSTR | ubiquitination | [1] | | 242 | LWRMSATKAWEVDTC | ubiquitination | [1] | | 275 | IISVGEDKTLRVWDL | ubiquitination | [1] | | 353 | IQSFNFQKRVASLPY | ubiquitination | [1] | | 364 | SLPYASLKGIGQPWD | ubiquitination | [1] | | 446 | EVRSLENKVTRNIKV | ubiquitination | [1] | | 610 | FKKALVNKNFPEVLR | acetylation | [2] | | 671 | VALDEAKKLNDSSTW | ubiquitination | [1] | | 722 | GDVNKLSKMQNIAQT | ubiquitination | [1] | | 812 | VISKPLEKWPLKEAE | acetylation | [2] | | 1189 | DRIAMISKIGAPASG | ubiquitination | [1] |
|
Reference | [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation. Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J. Nat Methods. 2013 Jul;10(7):676-82. [ PMID: 23749301] [2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae. Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C. Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [ PMID: 22865919] |
Functional Description | The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non- clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. |
Sequence Annotation | REPEAT 9 39 WD 1.
REPEAT 51 81 WD 2.
REPEAT 93 123 WD 3.
REPEAT 135 165 WD 4.
REPEAT 207 237 WD 5.
REPEAT 251 281 WD 6. |
Keyword | 3D-structure; Complete proteome; Cytoplasm; Cytoplasmic vesicle; Direct protein sequencing; ER-Golgi transport; Golgi apparatus; Membrane; Protein transport; Reference proteome; Repeat; Transport; WD repeat. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 1201 AA |
Protein Sequence | MKMLTKFESK STRAKGIAFH PSRPWVLVAL FSSTIQLWDY RMGTLLHRFE DHEGPVRGLD 60
FHPTQPIFVS AGDDYTIKVW SLDTNKCLYT LTGHLDYVRT VFFHRELPWI ISASDDQTIR 120
IWNWQNRKEI ACLTGHNHFV MCAQFHPTDD LIVSASLDET IRIWDISGLR KRHSAPGTSS 180
FEEQMSAQQN LLDGSLGDCV VKFILEGHTR GVNWASFHPT LPLIVSGSDD RQVKLWRMSA 240
TKAWEVDTCR GHTNNVDSVI FHPHQNLIIS VGEDKTLRVW DLDKRTPVKQ FKRENDRFWL 300
IAAHPHINLF GAAHDSGIMV FKLDRERPCS FIHQNQLFFV NAEKQIQSFN FQKRVASLPY 360
ASLKGIGQPW DAFRSISYNP SQHSVLVNEA NGKFALVILP KQPVGAVEPT SVTQDTGNFA 420
TFVGRNRFVV YNKNTESVEV RSLENKVTRN IKVEETVRTI VAAGPGSVLV IHPREVILYD 480
VQQGKKVSQL AVKNVKYVSW SLDGQYVALM SKHTITLATK KLELINSMHE TIRIKSAAWD 540
ETGVLIYSTL NHIRYSLLNG DRGIIKTLEK TLYITKVQGK LVYCLNREGE IEILTIDPTE 600
YRFKKALVNK NFPEVLRLIK DSNLVGQNII SYLQKSGYPE IALQFVQDPH IRFDLALEYG 660
NLDVALDEAK KLNDSSTWER LIQEALAQGN ASLAEMIYQT QHSFDKLSFL YLVTGDVNKL 720
SKMQNIAQTR EDFGSMLLNT FYNNSTKERS SIFAEGGSLP LAYAVAKANG DEAAASAFLE 780
QAEVDEQDVT LPDQMDASNF VQRPVISKPL EKWPLKEAEL SYFEKAVLGQ IDDLTIDDET 840
PAVNTTQEQE EPLGEENFND EDIGEDEGAW DLGDEDLDVG EELPEEVEQG EITSPAQEVE 900
TAIWIKNSKL PAVLVAAGAF DAAVQALSKQ VGVVKLEPLK KYFTNIYEGC RTYIPSTPCE 960
LPAQLGYVRA YDDTVSEDQI LPYVPGLDVV NEKMNEGYKN FKLNKPDIAI ECFREAIYRI 1020
TLLMVDDAED EKLAHKILET AREYILGLSI ELERRSLKEG NTVRMLELAA YFTKAKLSPI 1080
HRTNALQVAM SQHFKHKNFL QASYFAGEFL KIISSGPRAE QARKIKNKAD SMASDAIPID 1140
FDPYAKFDIC AATYKPIYED TPSVSDPLTG SKYVITEKDK IDRIAMISKI GAPASGLRIR 1200
V 1201 |
Gene Ontology | GO:0030126; C:COPI vesicle coat; IMP:SGD. GO:0005198; F:structural molecule activity; IEA:InterPro. GO:0043130; F:ubiquitin binding; IDA:SGD. GO:0006888; P:ER to Golgi vesicle-mediated transport; IMP:SGD. GO:0006886; P:intracellular protein transport; IEA:InterPro. GO:0006890; P:retrograde vesicle-mediated transport, Golgi to ER; IMP:SGD. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |