UniProt Accession

 P4HA2_HUMAN; O15460; D3DQ85; D3DQ86; Q8WWN0 

Genbank Protein ID

 U90441; AJ314859; AJ314859; AY358970; CH471062; CH471062; CH471062; CH471062; BC035813 

Genbank Nucleotide ID

 AAB71339.1; CAC85688.1; CAC85689.1; AAQ89329.1; EAW62341.1; EAW62342.1; EAW62343.1; EAW62346.1; AAH35813.1 

Protein Name

 Prolyl 4-hydroxylase subunit alpha-2 

Protein Synonyms/Alias

 4-PH alpha-2; Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-2 

Gene Name

 P4HA2 

Gene Synonyms/Alias

 UNQ290/PRO330 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
64	KIKSWANKMEALTSK	ubiquitination	[1]
71	KMEALTSKSAADAEG	ubiquitination	[1]
134	EDEIGAAKALMRLQD	ubiquitination	[1]
383	VASYRVSKSSWLEED	ubiquitination	[1]
443	RPFDSGLKTEGNRLA	ubiquitination	[1, 2, 3]

Reference

 [1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094] 

Functional Description

 Catalyzes the post-translational formation of 4- hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins. 

Sequence Annotation

 REPEAT 207 240 TPR.
 DOMAIN 412 520 Fe2OG dioxygenase.
 METAL 430 430 Iron (By similarity).
 METAL 432 432 Iron (By similarity).
 METAL 501 501 Iron (By similarity).
 BINDING 511 511 2-oxoglutarate (Potential).
 CARBOHYD 115 115 N-linked (GlcNAc...) (Potential).
 CARBOHYD 264 264 N-linked (GlcNAc...) (Potential).  

Keyword

 Alternative splicing; Complete proteome; Dioxygenase; Endoplasmic reticulum; Glycoprotein; Iron; Metal-binding; Oxidoreductase; Reference proteome; Signal; TPR repeat; Vitamin C. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 535 AA 

Protein Sequence

Gene Ontology

 GO:0005788; C:endoplasmic reticulum lumen; IEA:UniProtKB-SubCell.
 GO:0009055; F:electron carrier activity; TAS:UniProtKB.
 GO:0005506; F:iron ion binding; IEA:InterPro.
 GO:0031418; F:L-ascorbic acid binding; IEA:UniProtKB-KW.
 GO:0016702; F:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen; IEA:UniProtKB-KW.
 GO:0004656; F:procollagen-proline 4-dioxygenase activity; TAS:ProtInc.
 GO:0018401; P:peptidyl-proline hydroxylation to 4-hydroxy-L-proline; IEA:Compara. 

Interpro

 IPR005123; Oxoglu/Fe-dep_dioxygenase.
 IPR006620; Pro_4_hyd_alph.
 IPR013547; Pro_4_hyd_alph_N.
 IPR013026; TPR-contain_dom.
 IPR011990; TPR-like_helical.
 IPR019734; TPR_repeat. 

Pfam

 PF03171; 2OG-FeII_Oxy
 PF08336; P4Ha_N 

SMART

 SM00702; P4Hc 

PROSITE

 PS51471; FE2OG_OXY
 PS50005; TPR
 PS50293; TPR_REGION 

PRINTS